Structural analysis of the yeast SWI/SNF chromatin remodeling complex
Graduate School of Biomedical Sciences; Program in Gene Function and Expression; Program in Molecular Medicine
Medical Subject Headings
Chromatin; DNA-Binding Proteins; Image Processing, Computer-Assisted; Iodine Radioisotopes; Macromolecular Substances; Microscopy, Electron, Scanning Transmission; Models, Molecular; Molecular Weight; *Nuclear Proteins; Protein Conformation; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription Factors
Life Sciences | Medicine and Health Sciences
Elucidating the mechanism of ATP-dependent chromatin remodeling is one of the largest challenges in the field of gene regulation. One of the missing pieces in understanding this process is detailed structural information on the enzymes that catalyze the remodeling reactions. Here we use a combination of subunit radio-iodination and scanning transmission electron microscopy to determine the subunit stoichiometry and native molecular weight of the yeast SWI/SNF complex. We also report a three-dimensional reconstruction of yeast SWI/SNF derived from electron micrographs.
Rights and Permissions
Citation: Nat Struct Biol. 2003 Feb;10(2):141-5. Link to article on publisher's site
Nature structural biology
Smith, Corey Lewis; Horowitz-Scherer, Rachel A.; Flanagan, Joan Frances; Woodcock, Christopher L.; and Peterson, Craig L., "Structural analysis of the yeast SWI/SNF chromatin remodeling complex" (2003). GSBS Student Publications. 1128.