GSBS Student Publications

Title

Structural analysis of the yeast SWI/SNF chromatin remodeling complex

UMMS Affiliation

Graduate School of Biomedical Sciences; Program in Gene Function and Expression; Program in Molecular Medicine

Date

1-14-2003

Document Type

Article

Medical Subject Headings

Chromatin; DNA-Binding Proteins; Image Processing, Computer-Assisted; Iodine Radioisotopes; Macromolecular Substances; Microscopy, Electron, Scanning Transmission; Models, Molecular; Molecular Weight; *Nuclear Proteins; Protein Conformation; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription Factors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Elucidating the mechanism of ATP-dependent chromatin remodeling is one of the largest challenges in the field of gene regulation. One of the missing pieces in understanding this process is detailed structural information on the enzymes that catalyze the remodeling reactions. Here we use a combination of subunit radio-iodination and scanning transmission electron microscopy to determine the subunit stoichiometry and native molecular weight of the yeast SWI/SNF complex. We also report a three-dimensional reconstruction of yeast SWI/SNF derived from electron micrographs.

Rights and Permissions

Citation: Nat Struct Biol. 2003 Feb;10(2):141-5. Link to article on publisher's site

DOI of Published Version

10.1038/nsb888

Related Resources

Link to Article in PubMed

Journal Title

Nature structural biology

PubMed ID

12524530