GSBS Student Publications

Title

Probing SWI/SNF remodeling of the nucleosome by unzipping single DNA molecules

UMMS Affiliation

Graduate School of Biomedical Sciences; PetersonCornell University; Department of Molecular Medicine

Date

5-30-2006

Document Type

Article

Medical Subject Headings

Chromosomal Proteins, Non-Histone; DNA; Molecular Probes; Nucleosomes; Transcription Factors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Chromatin-remodeling enzymes can overcome strong histone-DNA interactions within the nucleosome to regulate access of DNA-binding factors to the genetic code. By unzipping individual DNA duplexes, each containing a uniquely positioned nucleosome flanked by long segments of DNA, we directly probed histone-DNA interactions. The resulting disruption-force signatures were characteristic of the types and locations of interactions and allowed measurement of the positions of nucleosomes with 2.6-base-pair (bp) precision. Nucleosomes remodeled by yeast SWI/SNF were moved bidirectionally along the DNA, resulting in a continuous position distribution. The characteristic distance of motion was approximately 28 bp per remodeling event, and each event occurred with a catalytic efficiency of 0.4 min(-1) per nM SWI/SNF. Remodeled nucleosomes had essentially identical disruption signatures to those of unremodeled nucleosomes, indicating that their overall structure remained canonical. These results impose substantial constraints on the mechanism of SWI/SNF remodeling.

Rights and Permissions

Citation: Nat Struct Mol Biol. 2006 Jun;13(6):549-54. Epub 2006 May 28. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Nature structural and molecular biology

PubMed ID

16732285