GSBS Student Publications

Title

Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules

UMMS Affiliation

Graduate School of Biomedical Sciences; Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology

Date

5-14-2003

Document Type

Article

Medical Subject Headings

1-Phosphatidylinositol 3-Kinase; Adipocytes; Androstadienes; Animals; Bacterial Proteins; Biological Transport; Cell Membrane; Cells, Cultured; Enzyme Inhibitors; Fibroblasts; Glucose Transporter Type 4; Insulin; Intracellular Membranes; Kinesin; Luminescent Proteins; Mice; Microtubules; Molecular Sequence Data; Monosaccharide Transport Proteins; *Muscle Proteins; Oligonucleotide Array Sequence Analysis; Rats; Recombinant Fusion Proteins; Signal Transduction; Transport Vesicles

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Insulin stimulates glucose uptake in muscle and adipose cells by mobilizing intracellular membrane vesicles containing GLUT4 glucose transporter proteins to the plasma membrane. Here we show in live cultured adipocytes that intracellular membranes containing GLUT4-yellow fluorescent protein (YFP) move along tubulin-cyan fluorescent protein-labeled microtubules in response to insulin by a mechanism that is insensitive to the phosphatidylinositol 3 (PI3)-kinase inhibitor wortmannin. Insulin increased by several fold the observed frequencies, but not velocities, of long-range movements of GLUT4-YFP on microtubules, both away from and towards the perinuclear region. Genomics screens show conventional kinesin KIF5B is highly expressed in adipocytes and this kinesin is partially co-localized with perinuclear GLUT4. Dominant-negative mutants of conventional kinesin light chain blocked outward GLUT4 vesicle movements and translocation of exofacial Myc-tagged GLUT4-green fluorescent protein to the plasma membrane in response to insulin. These data reveal that insulin signaling targets the engagement or initiates the movement of GLUT4-containing membranes on microtubules via conventional kinesin through a PI3-kinase-independent mechanism. This insulin signaling pathway regulating KIF5B function appears to be required for GLUT4 translocation to the plasma membrane.

Rights and Permissions

Citation: EMBO J. 2003 May 15;22(10):2387-99. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

The EMBO journal

PubMed ID

12743033