GSBS Student Publications

Title

DNA immunization for influenza virus: studies using hemagglutinin- and nucleoprotein-expressing DNAs

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pathology

Date

8-1-1997

Document Type

Article

Medical Subject Headings

Animals; Antibodies, Viral; Biolistics; Female; Hemagglutinin Glycoproteins, Influenza Virus; Immunization; Influenza Vaccines; Lung; Mice; Mice, Inbred BALB C; Nucleoproteins; Orthomyxoviridae Infections; *RNA-Binding Proteins; Vaccines, DNA; Viral Core Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

DNA-based immunizations have been used to analyze the ability of DNA-expressed hemagglutinin (HA) and nucleoprotein (NP) to protect BALB/c mice against a homologous influenza virus, A/PR/8/34 (H1N1), challenge. The HA DNA, but not the NP DNA, protected mice against the lethal viral challenge. For the HA DNA, single gene gun inoculations of 0.04 microg and boosted inoculations of 0.004 microg of DNA raised complete protection. For the NP DNA, boosted gene gun immunizations of 0.4 microg of DNA and boosted intradermal or intramuscular injections of 50 microg of DNA failed to protect. The protection elicited by the HA DNA vaccine correlated with the titers of neutralizing antibody.

Rights and Permissions

Citation: J Infect Dis. 1997 Aug;176 Suppl 1:S50-5.

Related Resources

Link to Article in PubMed

Journal Title

The Journal of infectious diseases

PubMed ID

9240695