GSBS Student Publications

Title

Assessment of human CD4+ and CD8+ T lymphocyte responses in experimental viral vaccine studies

UMMS Affiliation

Graduate School of Biomedical Sciences; Center for Infectious Diseases and Vaccine Research

Date

12-17-1998

Document Type

Article

Medical Subject Headings

B-Lymphocytes; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cytotoxicity Tests, Immunologic; Cytotoxicity, Immunologic; Humans; Lymphocyte Activation; Quality Control; T-Lymphocytes, Cytotoxic; Viral Vaccines; Virus Diseases

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The traditional in vitro correlate of immunological protection is the induction by a vaccine of neutralizing antibodies against the virus. It was formerly assumed that protection induced by a vaccine was solely due to neutralizing antibodies. Neutralizing antibodies are potent in the prevention of certain diseases, but virus-specific CD4+ T helper cells aid in the proliferation of specific antigen-triggered B cells to make antibodies. CD8+ T cells are responsible for eliminating virus-infected cells during viral illness, and may act as a second line of defence by becoming activated and eliminating any cells that become infected despite the presence of neutralizing antibodies, for example because of a large challenge dose or antigenic variation at the antibody combining sites. We will briefly review our approaches for measuring human virus-specific CD4+ and CD8+ T cell responses to experimental vaccines. It is critically important to have sensitive, quality controlled assays, including positive controls. There are many potential variables in human T cell assays and pitfalls, which usually result in negative CTL results. Uninterpretable data are to be expected unless adequate preliminary testing has been done to establish sensitive, specific and controlled human antigen specific CD4+ and CD8+ T cell assays.

Rights and Permissions

Citation: Dev Biol Stand. 1998;95:95-104.

Related Resources

Link to Article in PubMed

Journal Title

Developments in biological standardization

PubMed ID

9855419

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