Biaxial failure properties of planar living tissue equivalents
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Physiology
Medical Subject Headings
Animals; *Biocompatible Materials; *Collagen; *Fibrin; Rats; Stress, Mechanical; *Tissue Engineering
Life Sciences | Medicine and Health Sciences
Quantification of the mechanical properties of living tissue equivalents (LTEs) is essential for assessing their ultimate functionality as tissue substitutes, yet their delicate nature makes failure testing problematic. For this study, we evaluated the validity of using an inflation device for quantifying the biaxial tensile failure properties of extremely delicate fibroblast-populated collagen gels (CGs) and fibrin gels (FGs). Small samples were circularly clamped and then inflated until rupture. Each sample assumed an approximately spherical shape and burst at its center indicating effective clamping. After two weeks in culture, all LTEs tested were fragile, but the FGs were significantly stronger and more extensible than the CGs (ultimate tensile strength 6.0 kPa +/- 2.0 kPa vs. 2.8 kPa +/- 0.7 kPa; failure strain 3.5 +/- 0.9 vs. 0.26 +/- 0.05, n = 4). After an additional 11 days of culture, the strength of the FGs increased significantly (26.5 kPa +/- 12.7 kPa), and the extensibility decreased (1.9 +/- 0.8, n = 3). This study demonstrates that subtle differences in the properties of LTEs can be measured using inflation methods with minimal sample handling and without having to grow the tissues into anchors or cut the specimens.
Rights and Permissions
Citation: J Biomed Mater Res A. 2005 May 1;73(2):182-91. Link to article on publisher's site
DOI of Published Version
Journal of biomedical materials research. Part A
Billiar, Kristen L.; Throm, Angela M.; and Frey, Margo Tilley, "Biaxial failure properties of planar living tissue equivalents" (2005). GSBS Student Publications. 101.