GSBS Student Publications

Title

Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine

UMMS Affiliation

Graduate School of Biomedical Sciences; Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Biology; Program in Molecular Medicine

Date

3-31-1995

Document Type

Article

Medical Subject Headings

Animals; Calcium-Calmodulin-Dependent Protein Kinases; purification; Cell Line; Cercopithecus aethiops; Enzyme Activation; Hela Cells; Humans; Inflammation; Interleukin-1; JNK Mitogen-Activated Protein Kinases; Lipopolysaccharides; Mitogen-Activated Protein Kinase 3; *Mitogen-Activated Protein Kinases; Molecular Weight; Osmolar Concentration; Phosphorylation; Phosphothreonine; Phosphotyrosine; Recombinant Proteins; Sequence Deletion; Stress; Subcellular Fractions; Substrate Specificity; *Threonine; Transfection; Tumor Necrosis Factor-alpha; *Tyrosine

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Protein kinases activated by dual phosphorylation on Tyr and Thr (MAP kinases) can be grouped into two major classes: ERK and JNK. The ERK group regulates multiple targets in response to growth factors via a Ras-dependent mechanism. In contrast, JNK activates the transcription factor c-Jun in response to pro-inflammatory cytokines and exposure of cells to several forms of environmental stress. Recently, a novel mammalian protein kinase (p38) that shares sequence similarity with mitogen-activated protein (MAP) kinases was identified. Here, we demonstrate that p38, like JNK, is activated by treatment of cells with pro-inflammatory cytokines and environmental stress. The mechanism of p38 activation is mediated by dual phosphorylation on Thr-180 and Tyr-182. Immunofluorescence microscopy demonstrated that p38 MAP kinase is present in both the nucleus and cytoplasm of activated cells. Together, these data establish that p38 is a member of the mammalian MAP kinase group.

Rights and Permissions

Citation: J Biol Chem. 1995 Mar 31;270(13):7420-6.

Related Resources

Link to article in PubMed

Journal Title

The Journal of biological chemistry

PubMed ID

7535770