GSBS Student Publications

Title

Multiple copies of the bone-specific osteocalcin gene in mouse and rat

UMMS Affiliation

Graduate School of Biomedical Sciences; Graduate School of Biomedical Sciences; Department of Cell Biology

Date

12-1-1993

Document Type

Article

Medical Subject Headings

Amino Acid Sequence; Animals; Base Sequence; Blotting, Southern; Bone and Bones; *Genes; Mice; Mice, Inbred Strains; Molecular Sequence Data; *Multigene Family; Osteocalcin; Rats; Rats, Inbred Strains

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The osteocalcin (OC) gene was initially described as a single copy gene encoding the bone specific vitamin K dependent and vitamin D regulated protein. We report here the presence of multiple copies of the gene in mouse and rat. Southern blot analysis and restriction mapping of genomic DNA from several strains of mice indicated the presence of at least three copies of the OC coding sequence within a 19 kb fragment. Two closely linked OC genes contain the proximal promoter region with intact coding sequences. The third potential OC gene includes a 3.5 kb insert between an OC promoter-like region and a coding region that has several amino acid substitutions distributed among functional domains when compared with the normal gene. The 940 nucleotides upstream of the modified coding region lack the well defined 5' regulatory elements that support basal and hormone-responsive transcriptional control. In rats either one or more OC genes were observed in different strains or in Sprague Dawley rats obtained from different suppliers.

Rights and Permissions

Citation: Endocrinology. 1993 Dec;133(6):3050-3.

Related Resources

Link to article in PubMed

Journal Title

Endocrinology

PubMed ID

8243336