GSBS Student Publications

Title

Alteration of T4 lysozyme structure by second-site reversion of deleterious mutations

UMMS Affiliation

Graduate School of Biomedical Sciences; Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Date

2-12-1998

Document Type

Article

Medical Subject Headings

Bacteriophage P22; Bacteriophage T4; Genes, Viral; Muramidase; *Mutation; Recombinant Proteins; Salmonella typhimurium; *Suppression, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Mutations that suppress the defects introduced into T4 lysozyme by single amino acid substitutions were isolated and characterized. Among 53 primary sites surveyed, 8 yielded second-site revertants; a total of 18 different mutants were obtained. Most of the restorative mutations exerted global effects, generally increasing lysozyme function in a number of primary mutant contexts. Six of them were more specific, suppressing only certain specific deleterious primary substitutions, or diminishing the function of lysozymes bearing otherwise nondeleterious primary substitutions. Some variants of proteins bearing primary substitutions at the positions of Asp 20 and Ala 98 are inferred to have significantly altered structures.

Rights and Permissions

Citation: Protein Sci. 1997 Nov;6(11):2418-25.

Related Resources

Link to article in PubMed

Journal Title

Protein science : a publication of the Protein Society

PubMed ID

9385644