Subnuclear distribution of the vitamin D receptor
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Cell Biology
Medical Subject Headings
Animals; Base Sequence; Cell Nucleus; DNA; DNA-Binding Proteins; Molecular Sequence Data; Nuclear Matrix; Nuclear Proteins; Osteosarcoma; Proto-Oncogene Proteins c-jun; Rats; Receptors, Calcitriol; Transcription Factors; Tumor Cells, Cultured; Vitamin D
Life Sciences | Medicine and Health Sciences
The subnuclear distribution of the vitamin D receptor was investigated to begin addressing the contribution of nuclear architecture to vitamin D-responsive control of gene expression in ROS 17/2.8 rat osteosarcoma cells. The nuclear matrix is an anastomosing network of filaments that is functionally associated with DNA replication, transcription, and RNA processing. The representation of vitamin D receptor in the nuclear matrix and nonmatrix nuclear fractions was determined by the combined application of 1) sequence-specific interactions with the vitamin D receptor binding element of the rat bone-specific osteocalcin gene promoter and 2) Western blot analysis. Both methods confirmed the presence of vitamin D receptor in the nonmatrix nuclear fraction and the absence of detectable vitamin D receptors associated with the nuclear matrix. In contrast, these same nuclear matrix proteins preparations exhibited association with the general transcription factor AP-1 and a bone tissue-specific promoter binding factor NMP2. NMP-2 exhibits recognition for a promoter domain contiguous to the vitamin D-responsive element of the osteocalcin gene, although the vitamin D receptor does not appear to be a component of the nuclear matrix proteins. Interrelationships between nuclear matrix proteins and nonmatrix nuclear proteins, in mediating steroid hormone responsiveness of a vitamin D-regulated promoter, are therefore suggested.
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Citation: J Cell Biochem. 1994 Apr;54(4):494-500. Link to article on publisher's site
Journal of cellular biochemistry
Bidwell, Joseph P.; Van Wijnen, Andre J.; Fey, Edward G.; Merriman, Harold L.; Penman, Sheldon; Stein, Janet L.; Stein, Gary S.; and Lian, Jane B., "Subnuclear distribution of the vitamin D receptor" (1994). GSBS Student Publications. 100.