GSBS Student Publications

Title

Subnuclear distribution of the vitamin D receptor

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology

Date

4-1-1994

Document Type

Article

Medical Subject Headings

Animals; Base Sequence; Cell Nucleus; DNA; DNA-Binding Proteins; Molecular Sequence Data; Nuclear Matrix; Nuclear Proteins; Osteosarcoma; Proto-Oncogene Proteins c-jun; Rats; Receptors, Calcitriol; Transcription Factors; Tumor Cells, Cultured; Vitamin D

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The subnuclear distribution of the vitamin D receptor was investigated to begin addressing the contribution of nuclear architecture to vitamin D-responsive control of gene expression in ROS 17/2.8 rat osteosarcoma cells. The nuclear matrix is an anastomosing network of filaments that is functionally associated with DNA replication, transcription, and RNA processing. The representation of vitamin D receptor in the nuclear matrix and nonmatrix nuclear fractions was determined by the combined application of 1) sequence-specific interactions with the vitamin D receptor binding element of the rat bone-specific osteocalcin gene promoter and 2) Western blot analysis. Both methods confirmed the presence of vitamin D receptor in the nonmatrix nuclear fraction and the absence of detectable vitamin D receptors associated with the nuclear matrix. In contrast, these same nuclear matrix proteins preparations exhibited association with the general transcription factor AP-1 and a bone tissue-specific promoter binding factor NMP2. NMP-2 exhibits recognition for a promoter domain contiguous to the vitamin D-responsive element of the osteocalcin gene, although the vitamin D receptor does not appear to be a component of the nuclear matrix proteins. Interrelationships between nuclear matrix proteins and nonmatrix nuclear proteins, in mediating steroid hormone responsiveness of a vitamin D-regulated promoter, are therefore suggested.

Rights and Permissions

Citation: J Cell Biochem. 1994 Apr;54(4):494-500. Link to article on publisher's site

DOI of Published Version

10.1002/jcb.240540417

Related Resources

Link to article in PubMed

Journal Title

Journal of cellular biochemistry

PubMed ID

8014199