Title

Neuroprotective effect of hyperbaric oxygen therapy monitored by MR-imaging after embolic stroke in rats

Student Author(s)

Kenneth Sicard

UMMS Affiliation

Department of Neurology; Graduate School of Biomedical Sciences, MD/PhD Program

Date

7-4-2006

Document Type

Article

Medical Subject Headings

Animals; Arterial Occlusive Diseases; Brain Infarction; Brain Ischemia; Cerebral Arterial Diseases; Cerebral Cortex; Embolism; *Hyperbaric Oxygenation; Magnetic Resonance Imaging; Male; Middle Cerebral Artery; Neuroprotective Agents; Oxygen; Rats; Rats, Wistar; Stroke; Survival Analysis; Time Factors

Abstract

The potential neuroprotective effects of hyperbaric oxygen (HBO) were tested in an embolic model of focal cerebral ischemia with partially spontaneous reperfusion. Rats (n = 10) were subjected to embolic middle cerebral artery occlusion (MCAO) and diffusion weighted MRI (DWI) was performed at baseline, 1, 3, and 6 h after MCAO to determine the ADC viability threshold yielding the lesion volumes that best approximated the 2,3,5-triphenyltetrazolium chloride (TTC) infarct volumes at 24 h (experiment 1). For assessment of neuroprotective effects, rats were treated with 100% oxygen at 2.5 atmospheres absolute (ATA, n = 15) or normobaric room air (n = 15) for 60 min beginning 180 min after MCAO (experiment 2). DWI-, perfusion (PWI)- and T2-weighted MRI (T2WI) started within 0.5 h after MCAO and was continued 5 h, 24 h (PWI and T2WI only), and 168 h (T2WI only). Infarct volume was calculated based on TTC-staining at 24 h (experiment 1) or 168 h (experiment 2) post-MCAO. ADC-lesion evolution was maximal between 3 and 6 h. In experiment 2, the relative regional cerebral blood volume (rCBV) of both groups showed similar incomplete spontaneous reperfusion in the ischemic core. HBO reduced infarct volume to 145.3 +/- 39.6 mm3 vs. 202.5 +/- 58.3 mm3 (control, P = 0.029). As shown by MRI and TTC, HBO treatment demonstrated significant neuroprotection at 5 h after embolic focal cerebral ischemia that lasted for 168 h.

Rights and Permissions

Citation: Exp Neurol. 2006 Oct;201(2):316-23. Epub 2006 Jun 30. Link to article on publisher's site

Comments

Co-author Kenneth Sicard is a doctoral student in the Md/PhD Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Related Resources

Link to Article in PubMed

PubMed ID

16814772