Interdisciplinary Graduate Program
Program in Molecular Medicine
First Thesis Advisor
William Theurkauf, PhD
Caenorhabditis elegans, DNA Transposable Elements, Gene Silencing, Genome, Cell Cycle Proteins
Theses, UMMS; Caenorhabditis elegans; DNA Transposable Elements; Gene Silencing; Genome; Cell Cycle Proteins
Mobile genetic elements represent a large portion of the genome in many species. Posing a danger to the integrity of genetic information, silencing and structural machinery has evolved to suppress the mobility of foreign and transposable elements within the genome. Condensin proteins – which regulate chromosome structure to promote chromosome segregation – have been demonstrated to function in repetitive gene regulation and transposon silencing in several species. In model system Caenorhabditis elegans, microarray analysis studies have implicated Condensin II subunit HCP-6 in the silencing of multiple loci, including DNA transposon MIRAGE. To address the hypothesis that HCP-6 has a direct function in transcriptional gene silencing of the MIRAGE transposon, we queried MIRAGE expression and chromatin profiles in wild-type and hcp-6 mutant animals. Our evidence confirms that HCP-6 does indeed function during silencing of MIRAGE. However, we found no significant indication that HCP-6 binds to MIRAGE, nor that HCP-6 mediates MIRAGE enrichment of H3K9me3, the repressive heterochromatin mark observed at regions undergoing transcriptional silencing. We suggest that the silencing of MIRAGE, a newly evolved transposon and the only tested mobile element considerably derepressed upon loss of HCP-6, is managed by HCP-6 indirectly.
Malinkevich, A. MIRAGE DNA Transposon Silencing by C. elegans Condensin II Subunit HCP-6: A Masters Thesis. (2014). University of Massachusetts Medical School. GSBS Dissertations and Theses. Paper 754. DOI: 10.13028/M2TG72. http://escholarship.umassmed.edu/gsbs_diss/754
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