Graduate School of Biomedical Sciences, MS in Clinical Investigation Program
Theses, UMMS; MicroRNAs; Acetaminophen
To investigate plasma microRNA (miRNA) profiles indicative of hepatotoxicity in the setting of lethal acetaminophen (APAP) toxicity in mice.
Using plasma from APAP poisoned mice, either lethally (500 mg/kg) or sublethally (150 mg/kg) dosed, we screened commercially available murine microRNA libraries (SABiosciences, Qiagen Sciences, MD) to evaluate for unique miRNA profiles between these two dosing parameters.
We distinguished numerous, unique plasma miRNAs both up- and down-regulated in lethally compared to sublethally dosed mice. Of note, many of the greatest up- and down-regulated miRNAs, included, but were not limited to, 574-5p, 466g, 466f-3p, 375, 29c, and 148a. There was a statistically significant increase in alanine aminotransferase levels in the lethal compared to sublethal APAP dosing groups at the 12 h time point ( P < 0.001). There was 90% mortality in the lethally compared to sublethally dosed mice at the 48 h time point ( P = 0.011).
We identified unique plasma miRNAs both up- and down-regulated in lethally dosed APAP poisoned mice.
Ward, J. MicroRNA Markers of Acetaminophen Toxicity: A Master's Thesis. (2012). University of Massachusetts Medical School. GSBS Dissertations and Theses. Paper 625. http://escholarship.umassmed.edu/gsbs_diss/625
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