Human monocytes, macrophages, and dendritic cells: alcohol treatment methods
Department of Medicine, Division of Gastroenterology; Department of Medicine, Rheumatology Division
Medical Subject Headings
*Cell Culture Techniques; Cell Separation; Cells, Cultured; Cytokines; Dendritic Cells; Dose-Response Relationship, Drug; Electrophoretic Mobility Shift Assay; Ethanol; Humans; Immunity, Innate; Lymphocyte Culture Test, Mixed; Macrophages; Monocytes; NF-kappa B
Biochemistry, Biophysics, and Structural Biology | Gastroenterology | Immunology and Infectious Disease | Molecular Biology
Both acute and chronic alcohol consumption have significant immunomodulatory effects of which alterations in innate immune functions contribute to impaired antimicrobial defense and inflammatory responses. Blood monocytes, macrophages, and dendritic cells play a central role in innate immune recognition as these cells recognize pathogens, respond with inflammatory cytokine production, and induce antigen-specific T-lymphocyte activation. All of these innate immune cell functions are affected in humans by alcohol intake. Here, we summarize the different effects of acute and chronic alcohol on monocyte, macrophage, and dendritic cell functions in humans and describe methods for separation and functional evaluation of these cell types.
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Citation: Methods Mol Biol. 2008;447:113-24. Link to article on publisher's site