Title

Role of elevated monocyte transforming growth factor beta (TGF beta) production in posttrauma immunosuppression

UMMS Affiliation

Department of Surgery; Department of Medicine, Division of Gastroenterology

Date

3-1-1991

Document Type

Article

Medical Subject Headings

Acetylmuramyl-Alanyl-Isoglutamine; Adult; Aged; Burns; Dinoprostone; Female; Humans; Immune Tolerance; Interferon-gamma; Male; Middle Aged; Monocytes; Transforming Growth Factor beta; Wounds and Injuries

Disciplines

Gastroenterology | Immunology and Infectious Disease

Abstract

We previously reported that increased production of prostaglandin E2 by monocytes is a pivotal mechanism in posttrauma immunopathology. Here we characterize monocyte levels of transforming growth factor beta and examine the effects of elevated transforming growth factor beta on prostaglandin E2 release by patients' monocytes. Trauma patients' and normals' monocyte supernates (+/- stimulation with muramyl dipeptide) were acid treated and assayed for transforming growth factor beta using the mink lung-cell bioassay. Alternatively, human transforming growth factor beta was added to patients' and normals' monocytes and prostaglandin E2 production assayed. Significantly elevated transforming growth factor beta levels (median = 181.7 pmol/10(6) monocytes) were detected in immunosuppressed patients' monocytes but not immunocompetent trauma patients' (median = 32.0 pM) or normals' (median = 20.4 pM) monocytes. Adding transforming growth factor beta to monocytes resulted in a significant elevation of prostaglandin E2 levels. Elevated monocyte transforming growth factor beta levels in trauma patients could be both suppressing T-lymphocyte functions and maintaining elevated monocyte prostaglandin E2 synthesis.

Rights and Permissions

Citation: J Clin Immunol. 1991 Mar;11(2):95-102.

Related Resources

Link to Article in PubMed

PubMed ID

1905306