Advanced molecular biologic techniques in toxicologic disease
Department of Emergency Medicine; Department of Medicine, Division of Cardiovascular Medicine; Department of Medicine, Division of Gastroenterology; Meyers Primary Care Institute
Medical Subject Headings
*Drug Toxicity; Gene Expression Profiling; Genomics; Humans; Metabolomics; MicroRNAs; Molecular Biology; Proteomics; Systems Biology
Medical Toxicology | Molecular Biology | Toxicology
The advancement of molecular biologic techniques and their capabilities to answer questions pertaining to mechanisms of pathophysiologic events have greatly expanded over the past few years. In particular, these opportunities have provided researchers and clinicians alike the framework from with which to answer clinical questions not amenable for elucidation using previous, more antiquated methods. Utilizing extremely small molecules, namely microRNA, DNA, protein, and nanoparticles, we discuss the background and utility of these approaches to the progressive, practicing physician. Finally, we consider the application of these tools employed as future bedside point of care tests, aiding in the ultimate goal of unsurpassed patient care.