Department of Medicine, Division of Infectious Diseases and Immunology
Medical Subject Headings
Animals; Biolistics; Electroporation; Humans; Immunization; Injections; Vaccines, DNA
Immunoprophylaxis and Therapy
DNA immunization was discovered in early 1990s, and its use has been expanded from vaccine studies to a broader range of biomedical research areas, such as the generation of high-quality polyclonal and monoclonal antibodies as research reagents. In this unit, three common DNA immunization methods are described: needle injection, electroporation, and gene gun. In addition, several common considerations related to DNA immunization are discussed.
Rights and Permissions
Citation: Curr Protoc Microbiol. 2013 Nov 5;31:18.3.1-18.3.24. doi:10.1002/9780471729259.mc1803s31. Link to article on publisher's site
DNA vaccine, electroporation, gene gun, immunization
Wang, Shixia and Lu, Shan, "DNA immunization" (2013). University of Massachusetts Medical School Faculty Publications. 790.