University of Massachusetts Medical School Faculty Publications

Title

DNA immunization

UMMS Affiliation

Department of Medicine, Division of Infectious Diseases and Immunology

Date

11-5-2013

Document Type

Article

Medical Subject Headings

Animals; Biolistics; Electroporation; Humans; Immunization; Injections; Vaccines, DNA

Disciplines

Immunoprophylaxis and Therapy

Abstract

DNA immunization was discovered in early 1990s, and its use has been expanded from vaccine studies to a broader range of biomedical research areas, such as the generation of high-quality polyclonal and monoclonal antibodies as research reagents. In this unit, three common DNA immunization methods are described: needle injection, electroporation, and gene gun. In addition, several common considerations related to DNA immunization are discussed.

Rights and Permissions

Citation: Curr Protoc Microbiol. 2013 Nov 5;31:18.3.1-18.3.24. doi:10.1002/9780471729259.mc1803s31. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Keywords

DNA vaccine, electroporation, gene gun, immunization

PubMed ID

24510291