Potent monoclonal antibodies against Clostridium difficile toxin A elicited by DNA immunization
Department of Medicine, Division of Infectious Diseases and Immunology
Animals; Antibodies, Bacterial; Antibodies, Monoclonal; Bacterial Toxins; Enterotoxins; Immunization; Mice, Inbred BALB C; Vaccines, DNA
Biological Factors | Genetics and Genomics | Immunology and Infectious Disease | Immunology of Infectious Disease | Immunoprophylaxis and Therapy
Recent studies have demonstrated that DNA immunization is effective in eliciting antigen-specific antibody responses against a wide range of infectious disease targets. The polyclonal antibodies elicited by DNA vaccination exhibit high sensitivity to conformational epitopes and high avidity. However, there have been limited reports in literature on the production of monoclonal antibodies (mAb) by DNA immunization. Here, by using Clostridium difficile (C. diff) toxin A as a model antigen, we demonstrated that DNA immunization was effective in producing a panel of mAb that are protective against toxin A challenge and can also be used as sensitive reagents to detect toxin A from various testing samples. The immunoglobulin (Ig) gene usage for such mAb was also investigated. Further studies should be conducted to fully establish DNA immunization as a unique platform to produce mAb in various hosts.
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Citation: Hum Vaccin Immunother. 2013 Oct;9(10):2157-64. doi: 10.4161/hv.25656. Epub 2013 Jul 12. Link to article on publisher's site
Human vaccines and immunotherapeutics
Zhang, Chunhua; Jin, Ke; Xiao, Yanling; Cheng, Ying; Huang, Zuhu; Wang, Shixia; and Lu, Shan, "Potent monoclonal antibodies against Clostridium difficile toxin A elicited by DNA immunization" (2013). University of Massachusetts Medical School Faculty Publications. 789.