Delineating breast ductal carcinoma using combined dye-enhanced wide-field polarization imaging and optical coherence tomography
Department of Pathology
Adult; Aged; Carcinoma, Ductal, Breast; Coloring Agents; Female; Humans; Intraoperative Period; Middle Aged; *Molecular Imaging; *Tomography, Optical Coherence
Investigative Techniques | Medical Biophysics | Neoplasms | Pathology
Intra-operative delineation of breast cancer is a challenging problem. We used dye-enhanced wide-field polarization imaging for rapid demarcation of en face cancer margins and optical coherence tomography (OCT) for cross-sectional evaluation. Ductal carcinoma specimens were stained with methylene blue. Wide-field reflectance images were acquired at 440 and 640 nm. Wide-field fluorescence images were excited at 640 nm and registered between 660 nm and 750 nm. OCT images were acquired using a 1310 nm swept-source system. The results were validated against histopathology. Both imaging modalities provided diagnostic information on cancer margins. Combined OCT and wide-field polarization imaging shows promise for intra-operative detection of ductal breast carcinoma.
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Citation: J Biophotonics. 2013 Sep;6(9):679-86. doi: 10.1002/jbio.201200102. Epub 2012 Sep 25. Link to article on publisher's site
Journal of biophotonics
Patel, Rakesh; Khan, Ashraf; Kamionek, Michal; Kandil, Dina; Quinlan, Robert; and Yaroslavsky, Anna N., "Delineating breast ductal carcinoma using combined dye-enhanced wide-field polarization imaging and optical coherence tomography" (2013). University of Massachusetts Medical School Faculty Publications. 593.