University of Massachusetts Medical School Faculty Publications


Delineating breast ductal carcinoma using combined dye-enhanced wide-field polarization imaging and optical coherence tomography

UMMS Affiliation

Department of Pathology

Publication Date


Document Type



Adult; Aged; Carcinoma, Ductal, Breast; Coloring Agents; Female; Humans; Intraoperative Period; Middle Aged; *Molecular Imaging; *Tomography, Optical Coherence


Investigative Techniques | Medical Biophysics | Neoplasms | Pathology


Intra-operative delineation of breast cancer is a challenging problem. We used dye-enhanced wide-field polarization imaging for rapid demarcation of en face cancer margins and optical coherence tomography (OCT) for cross-sectional evaluation. Ductal carcinoma specimens were stained with methylene blue. Wide-field reflectance images were acquired at 440 and 640 nm. Wide-field fluorescence images were excited at 640 nm and registered between 660 nm and 750 nm. OCT images were acquired using a 1310 nm swept-source system. The results were validated against histopathology. Both imaging modalities provided diagnostic information on cancer margins. Combined OCT and wide-field polarization imaging shows promise for intra-operative detection of ductal breast carcinoma.

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Citation: J Biophotonics. 2013 Sep;6(9):679-86. doi: 10.1002/jbio.201200102. Epub 2012 Sep 25. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Journal of biophotonics

PubMed ID