DNA Replication Timing
Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
DNA Replication Timing; Eukaryota; Genome
Genomics | Molecular Genetics | Structural Biology
Patterns of replication within eukaryotic genomes correlate with gene expression, chromatin structure, and genome evolution. Recent advances in genome-scale mapping of replication kinetics have allowed these correlations to be explored in many species, cell types, and growth conditions, and these large data sets have allowed quantitative and computational analyses. One striking new correlation to emerge from these analyses is between replication timing and the three-dimensional structure of chromosomes. This correlation, which is significantly stronger than with any single histone modification or chromosome-binding protein, suggests that replication timing is controlled at the level of chromosomal domains. This conclusion dove tails with parallel work on the heterogeneity of origin firing and the competition between origins for limiting activators to suggest a model in which the stochastic probability of individual origin firing is modulated by chromosomal domain structure to produce patterns of replication. Whether these patterns have inherent biological functions or simply reflect higher-order genome structure is an open question.
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Citation: Cold Spring Harb Perspect Med. 2013 Jul;3(7):1-26. doi: 10.1101/cshperspect.a010132
Rhind, Nicholas R. and Gilbert, David M., "DNA Replication Timing" (2013). University of Massachusetts Medical School Faculty Publications. 489.