Department of Microbiology and Physiological Systems
Medical Subject Headings
Amino Acid Substitution; HN Protein; Mutant Proteins; Newcastle disease virus; Protein Binding; *Protein Multimerization; Viral Fusion Proteins; *Virus Internalization
Newcastle disease virus (NDV)-induced membrane fusion requires formation of a complex between the hemagglutinin-neuraminidase (HN) and fusion (F) proteins. Substitutions for NDV HN stalk residues A89, L90, and L94 block fusion by modulating formation of the HN-F complex. Here, we demonstrate that a nearby L97A substitution, though previously shown to block fusion, allows efficient HN-F complex formation and likely acts by preventing changes in the HN stalk required for triggering of the bound F protein.
Mirza, Anne M. and Iorio, Ronald M., "A mutation in the stalk of the newcastle disease virus hemagglutinin-neuraminidase (HN) protein prevents triggering of the F protein despite allowing efficient HN-F complex formation" (2013). University of Massachusetts Medical School Faculty Publications. 385.