University of Massachusetts Medical School Faculty Publications

Title

Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules

UMMS Affiliation

Program in Molecular Medicine

Publication Date

11-2013

Document Type

Article

Disciplines

Cell and Developmental Biology

Abstract

Anterior foregut endoderm (AFE) gives rise to therapeutically relevant cell types in tissues such as the esophagus, salivary glands, lung, thymus, parathyroid and thyroid. Despite its importance, reports describing the generation of AFE from pluripotent stem cells (PSCs) by directed differentiation have mainly focused on the Nkx2.1(+) lung and thyroid lineages. Here, we describe a novel protocol to derive a subdomain of AFE, identified by expression of Pax9, from PSCs using small molecules and defined media conditions. We generated a reporter PSC line for isolation and characterization of Pax9(+) AFE cells, which when transplanted in vivo, can form several distinct complex AFE-derived epithelia, including mucosal glands and stratified squamous epithelium. Finally, we show that the directed differentiation protocol can be used to generate AFE from human PSCs. Thus, this work both broadens the range of PSC-derived AFE tissues and creates a platform enabling the study of AFE disorders.

Rights and Permissions

Citation: Kearns NA, Genga RM, Ziller M, Kapinas K, Peters H, Brehm MA, Meissner A, Maehr R. Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules. Stem Cell Res. 2013 Nov;11(3):1003-12. doi:10.1016/j.scr.2013.06.007.Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Stem cell research

PubMed ID

23917481