Improved detection rate of cytogenetic abnormalities in chronic lymphocytic leukemia and other mature B-cell neoplasms with use of CpG-oligonucleotide DSP30 and interleukin 2 stimulation
Department of Pathology; Department of Hospital Laboratories
Medical Subject Headings
Chromosome Aberrations; Cytogenetics; Interleukin-2; Leukemia, Lymphocytic, Chronic, B-Cell; Oligonucleotides
Diagnosis | Hemic and Lymphatic Diseases | Oncology | Pathology
Detection of cytogenetic abnormalities requires successful culture of the clonal population to obtain metaphase chromosomes for study, and as such, has been hampered by low mitotic indices of mature B cells in culture. Our study presents data on the improved abnormality detection rate with the use of a CpG-oligonucleotide/interleukin 2 (OL/IL-2) culture protocol for mature B-cell neoplasms, including chronic lymphocytic leukemia (CLL) and non-CLL specimens. The increased detection rate of abnormalities, compared with unstimulated culture and traditional pokeweed mitogen culture, was statistically significant for both CLL and non-CLL neoplasms. For CLL specimens, our data also showed that for cytogenetically visible aberrations, OL/IL-2 was as, if not more, sensitive than detection with interphase fluorescence in situ hybridization (iFISH). Use of OL/IL-2 allowed a number of abnormalities to be detected, which were not covered by specific iFISH panels, especially balanced translocations. Therefore, OL/IL-2 stimulation improves diagnostic sensitivity and increases discovery rate of novel prognostic findings.
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Citation: Am J Clin Pathol. 2013 May;139(5):662-9. doi: 10.1309/AJCP7G4VMYZJQVFI. Link to article on publisher's site
Shi, Min; Cipollini, Matthew J.; Crowley-Bish, Patricia; Higgins, Anne W.; Yu, Hongbo; and Miron, Patricia M., "Improved detection rate of cytogenetic abnormalities in chronic lymphocytic leukemia and other mature B-cell neoplasms with use of CpG-oligonucleotide DSP30 and interleukin 2 stimulation" (2013). University of Massachusetts Medical School Faculty Publications. 35.