University of Massachusetts Medical School Faculty Publications

Title

Improved detection rate of cytogenetic abnormalities in chronic lymphocytic leukemia and other mature B-cell neoplasms with use of CpG-oligonucleotide DSP30 and interleukin 2 stimulation

UMMS Affiliation

Department of Pathology; Department of Hospital Laboratories

Publication Date

5-2013

Document Type

Article

Subjects

Chromosome Aberrations; Cytogenetics; Interleukin-2; Leukemia, Lymphocytic, Chronic, B-Cell; Oligonucleotides

Disciplines

Diagnosis | Hemic and Lymphatic Diseases | Oncology | Pathology

Abstract

Detection of cytogenetic abnormalities requires successful culture of the clonal population to obtain metaphase chromosomes for study, and as such, has been hampered by low mitotic indices of mature B cells in culture. Our study presents data on the improved abnormality detection rate with the use of a CpG-oligonucleotide/interleukin 2 (OL/IL-2) culture protocol for mature B-cell neoplasms, including chronic lymphocytic leukemia (CLL) and non-CLL specimens. The increased detection rate of abnormalities, compared with unstimulated culture and traditional pokeweed mitogen culture, was statistically significant for both CLL and non-CLL neoplasms. For CLL specimens, our data also showed that for cytogenetically visible aberrations, OL/IL-2 was as, if not more, sensitive than detection with interphase fluorescence in situ hybridization (iFISH). Use of OL/IL-2 allowed a number of abnormalities to be detected, which were not covered by specific iFISH panels, especially balanced translocations. Therefore, OL/IL-2 stimulation improves diagnostic sensitivity and increases discovery rate of novel prognostic findings.

Rights and Permissions

Citation: Am J Clin Pathol. 2013 May;139(5):662-9. doi: 10.1309/AJCP7G4VMYZJQVFI. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

American journal of clinical pathology

PubMed ID

23596118