Program in Molecular Medicine
Medical Subject Headings
Cell Line; HIV-1; Humans; Microscopy, Confocal; RNA, Viral; Staining and Labeling; Uridine; Viral Proteins; Virology; *Virus Uncoating
BACKGROUND: Uncoating of the HIV-1 core plays a critical role during early post-fusion stages of infection but is poorly understood. Microscopy-based assays are unable to easily distinguish between intact and partially uncoated viral cores.
RESULTS: In this study, we used 5-ethynyl uridine (EU) to label viral-associated RNA during HIV production. At early time points after infection with EU-labeled virions, the viral-associated RNA was stained with an EU-specific dye and was detected by confocal microscopy together with viral proteins. We observed that detection of the viral-associated RNA was specific for EU-labeled virions, was detected only after viral fusion with target cells, and occurred after an initial opening of the core. In vitro staining of cores showed that the opening of the core allowed the small molecule dye, but not RNase A or antibodies, inside. Also, staining of the viral-associated RNA, which is co-localized with nucleocapsid, decays over time after viral infection. The decay rate of RNA staining is dependent on capsid (CA) stability, which was altered by CA mutations or a small molecule inducer of HIV-1 uncoating. While the staining of EU-labeled RNA was not affected by inhibition of reverse transcription, the kinetics of core opening of different CA mutants correlated with initiation of reverse transcription. Analysis of the E45A CA mutant suggests that initial core opening is independent of complete capsid disassembly.
CONCLUSIONS: Taken together, our results establish a novel RNA accessibility-based assay that detects an early event in HIV-1 uncoating and can be used to further define this process.
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Citation: Xu H, Franks T, Gibson G, Huber K, Rahm N, De Castillia CS, Luban J, Aiken C, Watkins S, Sluis-Cremer N, Ambrose Z. Evidence for biphasic uncoating during HIV-1 infection from a novel imaging assay. Retrovirology. 2013 Jul 9;10:70. doi: 10.1186/1742-4690-10-70. Link to article on publisher's site
Xu, Hongzhan; Franks, Tamera; Gibson, Gregory; Huber, Kelly; Rahm, Nadia; De Castilla, Caterina Strambio; Luban, Jeremy; Aiken, Christopher; Watkins, Simon; Sluis-Cremer, Nicolas; and Ambrose, Zandrea, "Evidence for biphasic uncoating during HIV-1 infection from a novel imaging assay" (2013). University of Massachusetts Medical School Faculty Publications. Paper 332.