Department of Cell and Developmental Biology
Cell Biology | Developmental Biology | Genetics and Genomics | Molecular, Cellular, and Tissue Engineering
BACKGROUND: Adult human fibroblasts grown in low oxygen and with FGF2 supplementation have the capacity to tip the healing outcome of skeletal muscle injury - by favoring regeneration response in vivo over scar formation. Here, we compare the transcriptomes of control adult human dermal fibroblasts and induced regeneration-competent (iRC) fibroblasts to identify transcriptional changes that may be related to their regeneration competence.
RESULTS: We identified a unique gene-expression profile that characterizes FGF2-induced iRC fibroblast phenotype. Significantly differentially expressed genes due to FGF2 treatment were identified and analyzed to determine overrepresented Gene Ontology terms. Genes belonging to extracellular matrix components, adhesion molecules, matrix remodelling, cytoskeleton, and cytokines were determined to be affected by FGF2 treatment.
CONCLUSIONS: Transcriptome analysis comparing control adult human fibroblasts with FGF2-treated fibroblasts identified functional groups of genes that reflect transcriptional changes potentially contributing to their regeneration competence. This comparative transcriptome analysis should contribute new insights into genes that characterize cells with greater regenerative potential.
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Citation: Kashpur O, LaPointe D, Ambady S, Ryder EF, Dominko T. FGF2-induced effects on transcriptome associated with regeneration competence in adult human fibroblasts. BMC Genomics. 2013 Sep 26;14:656. doi: 10.1186/1471-2164-14-656. Link to article on publisher's site
Transcriptome, Human fibroblasts, Fibroblast growth factor (FGF2), Wound healing, Regeneration
Kashpur, Olga; Lapointe, David S.; Ambady, Sakthikumar; Ryder, Elizabeth F.; and Dominko, Tanja, "FGF2-induced effects on transcriptome associated with regeneration competence in adult human fibroblasts" (2013). University of Massachusetts Medical School Faculty Publications. 321.