Culturing mouse cerebellar granule neurons
Department of Microbiology and Physiological Systems
Cell Biology | Laboratory and Basic Science Research | Molecular and Cellular Neuroscience | Molecular Biology | Neuroscience and Neurobiology
The cerebellum plays an important role in motor control, motor skill acquisition, memory and learning among other brain functions. In rodents, cerebellar development continues after birth, characterized by the maturation of granule neurons. Cerebellar granule neurons (CGNs) are the most abundant neuronal type in the central nervous system, and they provide an excellent model for investigating molecular, -cellular, and physiological mechanisms underlying neuronal development as well as neural circuitry linked to behavior. Here we describe a procedure to isolate and culture CGNs from postnatal day 6 mice. These cultures can be used to examine numerous aspects of CGN differentiation, electrophysiology, and function.
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Citation: Methods Mol Biol. 2013;1018:49-59. doi: 10.1007/978-1-62703-444-9_5. Link to article on publisher's site
Selvakumar, Tharakeswari and Kilpatrick, Daniel L., "Culturing mouse cerebellar granule neurons" (2013). University of Massachusetts Medical School Faculty Publications. 234.