The N-terminus of vaccinia virus host range protein C7L is essential for function
Department of Pathology; Department of Medicine, Division of Infectious Diseases and Immunology
DNA Mutational Analysis; *Host Specificity; Recombination, Genetic; Vaccinia virus; Viral Proteins
Immunology and Infectious Disease | Immunoprophylaxis and Therapy | Medical Microbiology | Molecular Genetics | Virology
Vaccinia virus (VACV), a member of the Poxviridae family of large double-stranded DNA viruses, is being used as a smallpox vaccine as well as an expression vector for immunization against other infectious diseases and cancer. The host range of wild type VACV is very broad among mammalian cells. C7L is a host range gene identified in VACV and is well conserved in mammalian poxviruses except for parapoxviruses and molluscum contagiosum virus. The molecular mechanisms by which the C7L gene exerts host range function are not well understood. The C7L protein does not have any known conserved domains or show sequence similarity to cellular proteins or viral proteins other than the C7L homologs in mammalian poxviruses. We generated recombinant vaccinia viruses carrying deletion mutants of the C7L gene using NYVAC as a parental strain and found that the N-terminus is essential for host range function of C7L, which is consistent with a previous report that showed that homology among C7L homologs are greater near the N-terminus than the C-terminus.
Vaccinia virus, Host range gene, C7L, Poxvirus, Immunomodulatory gene
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Citation: Virus Genes. 2013 Feb;46(1):20-7. doi: 10.1007/s11262-012-0822-x. Link to article on publisher's site
Terajima, Masanori; Urban, Stina L.; and Leporati, Anita M., "The N-terminus of vaccinia virus host range protein C7L is essential for function" (2013). University of Massachusetts Medical School Faculty Publications. 201.