University of Massachusetts Medical School Faculty Publications

Title

Mechanisms of action of External Volume Expansion devices

UMMS Affiliation

Department of Surgery

Date

9-2013

Document Type

Article

Medical Subject Headings

Tissue Expansion; Adipose Tissue; Tissue Transplantation; Reconstructive Surgical Procedures

Disciplines

Plastic Surgery | Surgery

Abstract

BACKGROUND:: External volume expansion (EVE) by suction has been proposed to improve the survival of fat grafting by preparing the recipient site. In previous experimental work, EVE demonstrated the capacity to stimulate cell proliferation, vessel remodeling and adipogenesis. This study investigated possible mechanisms underlying these observed changes.

METHODS:: A miniaturized EVE device was applied to the dorsum of mice for 2 hours. Hypoxia during stimulation was assessed with pimonidazole hydrochloride, and tissue perfusion was measured for up to 2 days via hyperspectral imaging. Treated tissues were evaluated by microscopy for the presence of edema, inflammation, the effects on cell proliferation and vessel remodeling.

RESULTS:: EVE-treated tissues were grossly expanded with 2 hours of stimulation, developing a macroscopic swelling that slowly regressed over the course of hours following stimulus cessation. This gross swelling was reflective of histological signs of intense edema, persistent for at least 1 hour post-EVE. Tissues were hypoxic during stimulation, and hyperspectral imaging demonstrated decreased tissue content of both oxygenated- and deoxygenated-hemoglobin in the first hour after EVE release. The onset of inflammation was already apparent by the end of stimulation, and remained elevated through two days post-EVE. At this time point epidermal and dermal cell proliferation, as well as vascular density, were significantly increased.

CONCLUSIONS:: EVE sets in motion various mechanisms including mechanical stimulation, edema, ischemia and inflammation that over distinct time periods maintain an environment conducive to cell proliferation and angiogenesis, which can be elicited even by a single 2 hour EVE cycle.

Rights and Permissions

Citation: Plast Reconstr Surg. 2013 Sep;132(3):569-78. doi: 10.1097/PRS.0b013e31829ace30. Link to article on publisher's site

Comments

Medical student Brian Freniere participated in this study as part of the Senior Scholars research program at the University of Massachusetts Medical School.

Related Resources

Link to Article in PubMed

PubMed ID

23676966