University of Massachusetts Medical School Faculty Publications

Title

A genome wide RNA interference screening method to identify host factors that modulate influenza A virus replication

UMMS Affiliation

Department of Microbiology and Physiological Systems

Date

2-2013

Document Type

Article

Disciplines

Genetics and Genomics | Genomics | Molecular Biology | Molecular Genetics | Virology

Abstract

The use of genome wide RNA interference (RNAi) screens to investigate host-virals interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.

Rights and Permissions

Citation: Methods. 2013 Feb;59(2):217-24. doi: 10.1016/j.ymeth.2012.09.009. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Keywords

siRNA screening, Automated siRNA transfection, Reverse transfection, Functional genomics, Host-virus interactions, Influenza A virus, Whole genome screen

PubMed ID

23036328