University of Massachusetts Medical School Faculty Publications


A genome wide RNA interference screening method to identify host factors that modulate influenza A virus replication

UMMS Affiliation

Department of Microbiology and Physiological Systems

Publication Date


Document Type



Genetics and Genomics | Genomics | Molecular Biology | Molecular Genetics | Virology


The use of genome wide RNA interference (RNAi) screens to investigate host-virals interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.

Rights and Permissions

Citation: Methods. 2013 Feb;59(2):217-24. doi: 10.1016/j.ymeth.2012.09.009. Link to article on publisher's site

Related Resources

Link to Article in PubMed


siRNA screening, Automated siRNA transfection, Reverse transfection, Functional genomics, Host-virus interactions, Influenza A virus, Whole genome screen

Journal/Book/Conference Title

Methods (San Diego, Calif.)

PubMed ID