A genome wide RNA interference screening method to identify host factors that modulate influenza A virus replication
Department of Microbiology and Physiological Systems
Genetics and Genomics | Genomics | Molecular Biology | Molecular Genetics | Virology
The use of genome wide RNA interference (RNAi) screens to investigate host-virals interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.
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Citation: Methods. 2013 Feb;59(2):217-24. doi: 10.1016/j.ymeth.2012.09.009. Link to article on publisher's site
siRNA screening, Automated siRNA transfection, Reverse transfection, Functional genomics, Host-virus interactions, Influenza A virus, Whole genome screen
Methods (San Diego, Calif.)
Chin, Christopher R. and Brass, Abraham L., "A genome wide RNA interference screening method to identify host factors that modulate influenza A virus replication" (2013). University of Massachusetts Medical School Faculty Publications. 198.