Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation [preprint]
Authors
Demo, GabrielLoveland, Anna B.
Svidritskiy, Egor
Gamper, Howard B.
Hou, Ya-Ming
Korostelev, Andrei A.
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyRNA Therapeutics Institute
Document Type
PreprintPublication Date
2020-12-29Keywords
Molecular BiologymRNA
ribosomes
Molecular Biology
Nucleic Acids, Nucleotides, and Nucleosides
Structural Biology
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Show full item recordAbstract
Frameshifting of mRNA during translation provides a strategy to expand the coding repertoire of cells and viruses. Where and how in the elongation cycle +1-frameshifting occurs remains poorly understood. We captured six ∼3.5-Å-resolution cryo-EM structures of ribosomal elongation complexes formed with the GTPase elongation factor G (EF-G). Three structures with a +1-frameshifting-prone mRNA reveal that frameshifting takes place during translocation of tRNA and mRNA. Prior to EF-G binding, the pre-translocation complex features an in-frame tRNA-mRNA pairing in the A site. In the partially translocated structure with EF-G, the tRNA shifts to the +1-frame codon near the P site, whereas the freed mRNA base bulges between the P and E sites and stacks on the 16S rRNA nucleotide G926. The ribosome remains frameshifted in the nearly post-translocation state. Our findings demonstrate that the ribosome and EF-G cooperate to induce +1 frameshifting during mRNA translocation.Source
bioRxiv 2020.12.29.424751; doi: https://doi.org/10.1101/2020.12.29.424751. Link to preprint on bioRxiv.
DOI
10.1101/2020.12.29.424751Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29651Rights
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.Distribution License
http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1101/2020.12.29.424751
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Except where otherwise noted, this item's license is described as The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.