Program in Molecular Medicine
Medical Subject Headings
Dendritic Cells; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter
Neuroscience and Neurobiology
Long-term synaptic plasticity involves changes in the expression and membrane insertion of cell-surface proteins. Interestingly, the mRNAs encoding many cell-surface proteins are localized to dendrites, but whether dendritic protein synthesis is required for activity-induced surface expression of specific proteins is unknown. Herein, we used microfluidic devices to demonstrate that dendritic protein synthesis is necessary for activity-induced insertion of GluN2A-containing NMDA receptors in rat hippocampal neurons. Furthermore, visualization of activity-induced local translation of GluN2A mRNA and membrane insertion of GluN2A protein in dendrites was directly observed and shown to depend on a 3' untranslated region cytoplasmic polyadenylation element and its associated translation complex. These findings uncover a novel mechanism for cytoplasmic polyadenylation element-mediated posttranscriptional regulation of GluN2A mRNA to control NMDA receptor surface expression during synaptic plasticity.
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Citation: Swanger SA, He YA, Richter JD, Bassell GJ. Dendritic GluN2A synthesis mediates activity-induced NMDA receptor insertion. J Neurosci. 2013 May 15;33(20):8898-908. doi: 10.1523/JNEUROSCI.0289-13.2013. Link to article on publisher's site
Swanger, Sharon A.; He, Yuncan A.; Richter, Joel D.; and Bassell, Gary J., "Dendritic GluN2A Synthesis Mediates Activity-Induced NMDA Receptor Insertion" (2013). University of Massachusetts Medical School Faculty Publications. 18.