Authors
Rinaldi, Vera D.Donnard, Elisa
Rasmussen, Morten
Kucukural, Alper
Yukselen, Onur
Garber, Manuel
Sharma, Upasna
Rando, Oliver J.
UMass Chan Affiliations
Garber LabGraduate School of Biomedical Sciences
Program in Molecular Medicine
Program in Bioinformatics and Integrative Biology
Department of Biochemistry and Molecular Pharmacology
Document Type
Journal ArticlePublication Date
2020-07-30Keywords
developmentdevelopmental biology
genetics
genomics
mouse
reproduction
sperm maturation
Cell and Developmental Biology
Cells
Developmental Biology
Genetics and Genomics
UMCCTS funding
Metadata
Show full item recordAbstract
Following testicular spermatogenesis, mammalian sperm continue to mature in a long epithelial tube known as the epididymis, which plays key roles in remodeling sperm protein, lipid, and RNA composition. To understand the roles for the epididymis in reproductive biology, we generated a single-cell atlas of the murine epididymis and vas deferens. We recovered key epithelial cell types including principal cells, clear cells, and basal cells, along with associated support cells that include fibroblasts, smooth muscle, macrophages and other immune cells. Moreover, our data illuminate extensive regional specialization of principal cell populations across the length of the epididymis. In addition to region-specific specialization of principal cells, we find evidence for functionally specialized subpopulations of stromal cells, and, most notably, two distinct populations of clear cells. Our dataset extends on existing knowledge of epididymal biology, and provides a wealth of information on potential regulatory and signaling factors that bear future investigation.Source
Rinaldi VD, Donnard E, Gellatly K, Rasmussen M, Kucukural A, Yukselen O, Garber M, Sharma U, Rando OJ. An atlas of cell types in the mouse epididymis and vas deferens. Elife. 2020 Jul 30;9:e55474. doi: 10.7554/eLife.55474. PMID: 32729827; PMCID: PMC7426093. Link to article on publisher's site
DOI
10.7554/eLife.55474Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29571PubMed ID
32729827Related Resources
Rights
Copyright Rinaldi et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.7554/eLife.55474
Scopus Count
Except where otherwise noted, this item's license is described as Copyright Rinaldi et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.