Department of Microbiology and Physiological Systems
Medical Subject Headings
3' Untranslated Regions; Base Sequence; *DNA Damage; Polyadenylation; RNA Polymerase II; RNA, Fungal; RNA, Messenger; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription Elongation, Genetic; Transcription Initiation, Genetic; Ultraviolet Rays
Cellular and Molecular Physiology | Molecular Biology | Molecular genetics
Alternative polyadenylation (APA) is conserved in all eukaryotic cells. Selective use of polyadenylation sites appears to be a highly regulated process and contributes to human pathogenesis. In this article we report that the yeast RPB2 gene is alternatively polyadenylated, producing two mRNAs with different lengths of 3'UTR. In normally growing wild-type cells, polyadenylation preferentially uses the promoter-proximal poly(A) site. After UV damage transcription of RPB2 is initially inhibited. As transcription recovers, the promoter-distal poly(A) site is preferentially used instead, producing more of a longer form of RPB2 mRNA. We show that the relative increase in the long RPB2 mRNA is not caused by increased mRNA stability, supporting the preferential usage of the distal poly(A) site during transcription recovery. We demonstrate that the 3'UTR of RPB2 is sufficient for this UV-induced regulation of APA. We present evidence that while transcription initiation rates do not seem to influence selection of the poly(A) sites of RPB2, the rate of transcription elongation is an important determinant.
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Citation: Nucleic Acids Res. 2013 Mar 1;41(5):3104-14. doi: 10.1093/nar/gkt020. Link to article on publisher's site