Department of Microbiology and Physiological Systems; Program in Immunology and Virology
Medical Subject Headings
Electrophoretic Mobility Shift Assay; Nipah Virus; Polysaccharides; Protein Binding; Protein Interaction Mapping; Viral Envelope Proteins; *Virus Internalization
Microbial Physiology | Microbiology | Molecular Biology | Virology
The promotion of membrane fusion by most paramyxoviruses requires an interaction between the viral attachment and fusion (F) proteins to enable receptor binding by the former to trigger the activation of the latter for fusion. Numerous studies demonstrate that the F-interactive sites on the Newcastle disease virus (NDV) hemagglutinin-neuraminidase (HN) and measles virus (MV) hemagglutinin (H) proteins reside entirely within the stalk regions of those proteins. Indeed, stalk residues of NDV HN and MV H that likely mediate the F interaction have been identified. However, despite extensive efforts, the F-interactive site(s) on the Nipah virus (NiV) G attachment glycoprotein has not been identified. In this study, we have introduced individual N-linked glycosylation sites at several positions spaced at intervals along the stalk of the NiV G protein. Five of the seven introduced sites are utilized as established by a retardation of electrophoretic mobility. Despite surface expression, ephrinB2 binding, and oligomerization comparable to those of the wild-type protein, four of the five added N-glycans completely eliminate the ability of the G protein to complement the homologous F protein in the promotion of fusion. The most membrane-proximal added N-glycan reduces fusion by 80%. However, unlike similar NDV HN and MV H mutants, the NiV G glycosylation stalk mutants retain the ability to bind F, indicating that the fusion deficiency of these mutants is not due to prevention of the G-F interaction. These findings suggest that the G-F interaction is not mediated entirely by the stalk domain of G and may be more complex than that of HN/H-F.
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Citation: J Virol. 2013 Mar;87(6):3119-29. doi: 10.1128/JVI.03084-12. Epub 2013 Jan 2. Link to article on publisher's site
Zhu, Qiyun; Biering, Scott B.; Mirza, Anne M.; Grasseschi, Brittany; Mahon, Paul J.; Lee, Benhur; Aguilar, Hector C.; and Iorio, Ronald M., "Individual N-glycans added at intervals along the stalk of the Nipah virus G protein prevent fusion but do not block the interaction with the homologous F protein" (2013). University of Massachusetts Medical School Faculty Publications. 168.