An Estradiol-Inducible Promoter Enables Fast, Graduated Control of Gene Expression in Fission Yeast [preprint]
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDocument Type
PreprintPublication Date
2017-02-17Keywords
cell biologySchizosaccharomyces pombe
gene expression
estradiol
Cell Biology
Fungi
Genetic Phenomena
Hormones, Hormone Substitutes, and Hormone Antagonists
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Show full item recordAbstract
The fission yeast Schizosaccharomyces pombe lacks a diverse toolkit of inducible promoters for experimental manipulation. Available inducible promoters suffer from slow induction kinetics, limited control of expression levels and/or a requirement for defined growth medium. In particular, no S. pombe inducible promoter systems exhibit a linear dose response, which would allow expression to be tuned to specific levels. We have adapted a fast, orthogonal promoter system with a large dynamic range and a linear dose response, based on β-estradiol-regulated function of the human estrogen receptor, for use in S. pombe. We show that this promoter system, termed Z3EV, turns on quickly, can reach a maximal induction of 20 fold, and exhibits a linear dose response over its entire induction range, with few off target effects. We demonstrate the utility of this system by regulating the mitotic inhibitor Wee1 to create a strain in which cell size is regulated by β-estradiol concentration. This promoter system will be of great utility for experimentally regulating gene expression in fission yeast.Source
bioRxiv 092338; doi: https://doi.org/10.1101/092338. Link to preprint on bioRxiv service.
DOI
10.1101/092338Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29328Related Resources
Now published in Yeast doi: 10.1002/yea.3235.
Rights
The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.Distribution License
http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1101/092338
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Except where otherwise noted, this item's license is described as The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.