A Novel Population Of Natural Killer Cells Pplays A Critical Role in the Depletion of Splenic B2 B Cells During Experimental Africian Trypanosomiasis
Document Type
Poster AbstractPublication Date
2016-05-20Keywords
Trypanosoma bruceihuman sleeping sickness
nagana
splenic B cells
Animal Sciences
Immunology of Infectious Disease
Parasitic Diseases
Parasitology
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Show full item recordAbstract
Mice infected with Trypanosoma brucei, the causative agent of human sleeping sickness and a contributor to nagana in cattle, rapidly lose the capacity to mount VSG-specific antibody responses, and die with uncontrolled parasitemia. We have shown (Bockstal et al., 2011, PLOS Pathogens) that the loss of humoral immune competence in the infected mice results from depletion of developing and mature splenic B cells. We now report that T. brucei-induced splenic B cell depletion is dependent upon the presence of the pore forming molecule perforin which is present in the cytotoxic granules of cytotoxic T lymphocytes, natural killer T cells and natural killer cells, occurs in the absence of T cells (and natural killer T cells), i.e., in T cell receptor (αβγδ)-/- mice, but does not occur in intact mice that are depleted of natural killer (NK) cells by treatment with monoclonal antibody specific for the NK1.1 differentiation antigen. In the intact mice, B cells are deleted after remission of the first T. brucei parasitemic wave. At this time natural killer cells are expressing the cytotoxic granule marker CD107a, indicating that they have degranulated, executing their effector function. Moreover, in vitro assays show that B cells from T. brucei infected mice are killed by natural killer cells from uninfected C57BL/6 mice but not efficiently killed by CD107a positive natural killer cells isolated from infected mice, which may be functionally exhausted.DOI
10.13028/tss1-7k69Permanent Link to this Item
http://hdl.handle.net/20.500.14038/28052Rights
Copyright the Author(s)Distribution License
http://creativecommons.org/licenses/by-nc-sa/3.0/ae974a485f413a2113503eed53cd6c53
10.13028/tss1-7k69