Targeted Mutagenesis of a Therapeutic Human Monoclonal IgG1 Antibody Prevents Gelation at High Concentrations
Authors
Casaz, PaulBoucher, Elisabeth N.
Wollacott, Rachel
Ozturk, Sadettin S.
Thomas, William D. Jr.
Wang, Yan
Document Type
Poster AbstractPublication Date
2014-05-20Keywords
Amino Acids, Peptides, and ProteinsBiotechnology
Immunoprophylaxis and Therapy
Molecular Biology
Therapeutics
Translational Medical Research
Virology
Metadata
Show full item recordAbstract
A common challenge encountered during development of high concentration monoclonal antibody formulations is preventing self-association. Depending on the antibody and its formulation, self-association can be seen as aggregation, precipitation, opalescence or phase separation. Here we report on an unusual manifestation of self-association, formation of a semi-solid gel or “gelation”. Therapeutic monoclonal antibody C4 was isolated from human B cells based on its strong potency in neutralizing bacterial toxin in animal models. The purified antibody possessed the unusual property of forming a firm, opaque white gel when it was formulated at concentrations >40 mg/mL and the temperature wasDOI
10.13028/s238-t268Permanent Link to this Item
http://hdl.handle.net/20.500.14038/27940Notes
Abstract of poster presented at the 2014 UMass Center for Clinical and Translational Science Research Retreat, held on May 20, 2014 at the University of Massachusetts Medical School, Worcester, Mass.
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Copyright the Author(s)Distribution License
http://creativecommons.org/licenses/by-nc-sa/3.0/ae974a485f413a2113503eed53cd6c53
10.13028/s238-t268