Poster Presentations

Start Date

20-5-2014 12:30 PM

Description

Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) are highly reactive species which play crucial roles in many fundamental physiological processes including cellular signalling pathways. Over-production of these reactive species by various stimuli leads to cellular oxidative stress which is linked to various disease conditions. Therefore, the development of novel detection methods for ROS and RNS is of great interest and indispensable for monitoring the dynamic changes of ROS and RNS in cells and for elucidating their mechanisms of trafficking and connections to diseases. We have been recently developing various fluorescent sensors which can selectively detect metal ions, ROS or RNS species in live cells or animals. Our turn-on profluorescent sensors are capable of imaging oxidative stress promoted by metal and H2O2 (i.e. the Fenton Reaction conditions) in living cells (Chem Commun 2010); our highly selective and sensitive iron sensors can image the endogenous exchangeable iron pools and their dynamic changes with subcellular resolution in living neuronal cells (ChemBioChem 2012 and unpublished data), and so do our superoxide sensors (ChemBioChem 2012 and unpublished data). Moreover, we have recently developed nitric oxide (NO) sensors for molecular imaging of stimulated NO production in live cells with subcellular resolution as well as novel near infra red (NIR) sensors for NO imaging in live animals.

Comments

Abstract of poster presented at the 2014 UMass Center for Clinical and Translational Science Research Retreat, held on May 20, 2014 at the University of Massachusetts Medical School, Worcester, Mass.

Creative Commons License

Creative Commons Attribution-Noncommercial-Share Alike 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.

 
May 20th, 12:30 PM

Fluorescent Probes for Molecular Imaging of ROS/RNS Species in Living Systems

Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) are highly reactive species which play crucial roles in many fundamental physiological processes including cellular signalling pathways. Over-production of these reactive species by various stimuli leads to cellular oxidative stress which is linked to various disease conditions. Therefore, the development of novel detection methods for ROS and RNS is of great interest and indispensable for monitoring the dynamic changes of ROS and RNS in cells and for elucidating their mechanisms of trafficking and connections to diseases. We have been recently developing various fluorescent sensors which can selectively detect metal ions, ROS or RNS species in live cells or animals. Our turn-on profluorescent sensors are capable of imaging oxidative stress promoted by metal and H2O2 (i.e. the Fenton Reaction conditions) in living cells (Chem Commun 2010); our highly selective and sensitive iron sensors can image the endogenous exchangeable iron pools and their dynamic changes with subcellular resolution in living neuronal cells (ChemBioChem 2012 and unpublished data), and so do our superoxide sensors (ChemBioChem 2012 and unpublished data). Moreover, we have recently developed nitric oxide (NO) sensors for molecular imaging of stimulated NO production in live cells with subcellular resolution as well as novel near infra red (NIR) sensors for NO imaging in live animals.

 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.