%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Navonil Banerjee, Raja Bhattacharya, Michael Gorczyca, Kevin M. Collins, Michael M. Francis
Local neuropeptide signaling modulates serotonergic transmission to shape the temporal organization of C. elegans egg-laying behavior
www.plosgenetics.org
www.plosgenetics.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 10 0 R/Annots 11 0 R/Contents 12 0 R/TrimBox[0 0 612 792]>>
endobj
11 0 obj
[13 0 R 14 0 R 15 0 R 16 0 R 17 0 R 18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R 28 0 R]
endobj
13 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref001)>>
endobj
14 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref005)>>
endobj
15 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref006)>>
endobj
16 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref011)>>
endobj
17 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref012)>>
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref017)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref009)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref018)>>
endobj
21 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref020)>>
endobj
22 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref021)>>
endobj
23 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.g001)>>
endobj
24 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref009)>>
endobj
25 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref020)>>
endobj
26 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref022)>>
endobj
27 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006697.ref024)>>
endobj
28 0 obj
<>/Border[0 0 0]/A 29 0 R>>
endobj
29 0 obj
<>
endobj
12 0 obj
[30 0 R 31 0 R 32 0 R 33 0 R 34 0 R 35 0 R 36 0 R 37 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R 43 0 R 44 0 R]
endobj
30 0 obj
<>stream
q
0.955 g
0 w
199.9998 713.9999 m
575.9997 713.9999 l
575.9997 559.0003 l
199.9998 559.0003 l
f*
0.83 0.64 0.02 0 k
378.9354 445.9465 m
383.6409 445.9465 l
h
f*
388.8567 445.9465 m
393.6189 445.9465 l
h
f*
468.5669 380.9197 m
473.3291 380.9197 l
h
f*
478.5449 380.9197 m
488.0126 380.9197 l
h
f*
365.8394 354.8976 m
375.3638 354.8976 l
h
f*
380.5228 354.8976 m
390.0472 354.8976 l
h
f*
343.7858 198.9354 m
348.548 198.9354 l
h
f*
350.8157 198.9354 m
360.3402 198.9354 l
h
f*
365.4992 198.9354 m
375.0236 198.9354 l
h
f*
406.8283 107.9433 m
416.3528 107.9433 l
h
f*
513.1843 94.9039 m
539.5465 94.9039 l
h
f*
203.4142 81.9213 m
208.1764 81.9213 l
h
f*
210.3874 81.9213 m
219.9118 81.9213 l
h
f*
222.1795 81.9213 m
231.7039 81.9213 l
h
f*
236.863 81.9213 m
246.3874 81.9213 l
h
f*
0 g
1 j
1 J
10 0 0 10 209.9905 696.9826 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(behavior)Tj
3.7133 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(model)Tj
2.7439 0 Td
(system)Tj
/F8 1 Tf
2.9367 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.0658 0 Td
[()15()4()9()2( )16(\n)]TJ
/F7 1 Tf
2.9991 0 Td
(cycles)Tj
2.5511 0 Td
(between)Tj
3.532 0 Td
(clusters)Tj
3.2315 0 Td
(of)Tj
1.0148 0 Td
(egg-laying)Tj
4.3369 0 Td
(and)Tj
1.7348 0 Td
(pro-)Tj
-32.3657 -1.2982 Td
(longed)Tj
2.9366 0 Td
(inactive)Tj
3.3392 0 Td
(periods.)Tj
3.4186 0 Td
(This)Tj
1.9899 0 Td
(temporal)Tj
3.8381 0 Td
(organization)Tj
5.278 0 Td
(provides)Tj
3.651 0 Td
(for)Tj
1.3833 0 Td
(spatial)Tj
2.778 0 Td
(dispersal)Tj
3.7304 0 Td
(of)Tj
1.0148 0 Td
(eggs,)Tj
-33.3579 -1.2983 Td
(presumably)Tj
4.8812 0 Td
(benefiting)Tj
4.2576 0 Td
(progeny)Tj
3.5093 0 Td
(by)Tj
1.1792 0 Td
(limiting)Tj
3.4015 0 Td
(local)Tj
2.075 0 Td
(overcrowding)Tj
5.7713 0 Td
(and)Tj
1.7291 0 Td
(competition)Tj
5.1023 0 Td
(for)Tj
1.3833 0 Td
(food.)Tj
-33.2898 -1.3039 Td
(Here)Tj
2.2053 0 Td
(we)Tj
1.3266 0 Td
(uncover)Tj
3.4809 0 Td
(a)Tj
0.652 0 Td
(novel)Tj
2.3981 0 Td
(neuromodulatory)Tj
7.2963 0 Td
(mechanism)Tj
4.8416 0 Td
(that)Tj
1.7801 0 Td
(shapes)Tj
2.8403 0 Td
(the)Tj
1.474 0 Td
(timing)Tj
2.8857 0 Td
(of)Tj
1.0148 0 Td
(egg-lay-)Tj
-32.1957 -1.2983 Td
(ing)Tj
1.5023 0 Td
(behavior.)Tj
3.9345 0 Td
(Specifically,)Tj
4.9152 0 Td
(we)Tj
1.3266 0 Td
(find)Tj
1.8539 0 Td
(that)Tj
1.7744 0 Td
(neuromodulatory)Tj
7.2964 0 Td
(signaling)Tj
3.8211 0 Td
(from)Tj
2.2053 0 Td
(a)Tj
0.652 0 Td
(group)Tj
2.6078 0 Td
(of)Tj
1.0148 0 Td
(non-)Tj
-32.9043 -1.2982 Td
(neuronal)Tj
3.821 0 Td
(cells)Tj
1.9106 0 Td
(specifies)Tj
3.5433 0 Td
(transitions)Tj
4.4787 0 Td
(between)Tj
3.5206 0 Td
(active)Tj
2.5115 0 Td
(and)Tj
1.7234 0 Td
(inactive)Tj
3.3279 0 Td
(phases)Tj
2.8346 0 Td
(of)Tj
1.0091 0 Td
(egg-laying)Tj
4.3313 0 Td
(by)Tj
1.1736 0 Td
(reg-)Tj
-34.1856 -1.3039 Td
(ulating)Tj
3.0103 0 Td
(neurotransmitter)Tj
7.0866 0 Td
(release)Tj
2.9027 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.474 0 Td
(motor)Tj
2.7212 0 Td
(output)Tj
2.897 0 Td
(neurons)Tj
3.5036 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(egg-laying)Tj
4.337 0 Td
(circuit.)Tj
-32.6265 -1.2983 Td
(Advancing)Tj
4.575 0 Td
(our)Tj
1.6271 0 Td
(knowledge)Tj
4.5411 0 Td
(of)Tj
1.0148 0 Td
(how)Tj
1.9389 0 Td
(neuropeptides)Tj
5.9357 0 Td
(and)Tj
1.7291 0 Td
(other)Tj
2.3471 0 Td
(modulators)Tj
4.8188 0 Td
(act)Tj
1.3777 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(context)Tj
-32.4111 -1.2983 Td
(of)Tj
1.0147 0 Td
(the)Tj
1.4684 0 Td
(circuits)Tj
3.1634 0 Td
(in)Tj
1.0318 0 Td
(which)Tj
2.6532 0 Td
(they)Tj
1.9219 0 Td
(are)Tj
1.4514 0 Td
(endogenously)Tj
5.7599 0 Td
(released)Tj
3.4299 0 Td
(will)Tj
1.6668 0 Td
(be)Tj
1.1452 0 Td
(critical)Tj
2.9536 0 Td
(in)Tj
1.0375 0 Td
(ongoing)Tj
3.5263 0 Td
(efforts)Tj
2.7553 0 Td
(to)Tj
-34.9793 -1.2982 Td
(understand)Tj
4.7905 0 Td
(how)Tj
1.9389 0 Td
(alternate)Tj
3.6963 0 Td
(behavioral)Tj
4.3937 0 Td
(states,)Tj
2.6192 0 Td
(for)Tj
1.3889 0 Td
(example)Tj
3.549 0 Td
(those)Tj
2.3357 0 Td
(underlying)Tj
4.6035 0 Td
(mood)Tj
2.5795 0 Td
(or)Tj
-31.8952 -1.304 Td
(arousal,)Tj
3.3278 0 Td
(are)Tj
1.4457 0 Td
(encoded.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 516.0188 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Introduction)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 499.011 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Animals)Tj
3.5773 0 Td
(have)Tj
2.0636 0 Td
(robust)Tj
2.7892 0 Td
(mechanisms)Tj
5.2044 0 Td
(in)Tj
1.0375 0 Td
(place)Tj
2.2677 0 Td
(to)Tj
1.0205 0 Td
(shape)Tj
2.4831 0 Td
(their)Tj
2.1089 0 Td
(behavior)Tj
3.7134 0 Td
(in)Tj
1.0375 0 Td
(a)Tj
0.6519 0 Td
(manner)Tj
3.3676 0 Td
(that)Tj
1.7745 0 Td
(is)Tj
0.8504 0 Td
(benefi-)Tj
-33.9475 -1.3039 Td
(cial)Tj
1.5873 0 Td
(both)Tj
2.0637 0 Td
(for)Tj
1.3832 0 Td
(their)Tj
2.109 0 Td
(survival)Tj
3.3392 0 Td
(and)Tj
1.7291 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
1.474 0 Td
(survival)Tj
3.3336 0 Td
(of)Tj
1.0148 0 Td
(their)Tj
2.1089 0 Td
(progeny.)Tj
3.7304 0 Td
(In)Tj
1.1112 0 Td
(many)Tj
2.4774 0 Td
(cases)Tj
2.2224 0 Td
(behaviors)Tj
4.0705 0 Td
(are)Tj
-35.138 -1.2982 Td
(composed)Tj
4.3029 0 Td
(from)Tj
2.2054 0 Td
(discrete,)Tj
3.5432 0 Td
(opposing)Tj
3.9118 0 Td
(behavioral)Tj
4.3937 0 Td
(states.)Tj
2.6192 0 Td
(Examples)Tj
4.0592 0 Td
(of)Tj
1.0148 0 Td
(behaviors)Tj
4.0705 0 Td
(that)Tj
1.7802 0 Td
(are)Tj
1.4456 0 Td
(organized)Tj
-33.3465 -1.2983 Td
(in)Tj
1.0318 0 Td
(this)Tj
1.6781 0 Td
(manner)Tj
3.3618 0 Td
(range)Tj
2.4661 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.4683 0 Td
(motor)Tj
2.7213 0 Td
(programs)Tj
4.0705 0 Td
(underlying)Tj
4.5978 0 Td
(feeding)Tj
3.1634 0 Td
(or)Tj
1.0998 0 Td
(locomotion)Tj
4.8529 0 Td
(to)Tj
1.0262 0 Td
(more)Tj
-33.7434 -1.2983 Td
(complex)Tj
3.6226 0 Td
(behaviors)Tj
4.0705 0 Td
(such)Tj
2.0636 0 Td
(as)Tj
1.0148 0 Td
(mood)Tj
2.5795 0 Td
(or)Tj
1.0999 0 Td
(arousal)Tj
3.1011 0 Td
([)Tj
0.83 0.64 0.02 0 k
(1)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
(].)Tj
2.5908 0 Td
(In)Tj
1.1112 0 Td
(each)Tj
2.0296 0 Td
(of)Tj
1.0148 0 Td
(these)Tj
2.2507 0 Td
(cases,)Tj
2.4491 0 Td
(animals)Tj
3.3278 0 Td
(adapt)Tj
2.4322 0 Td
(their)Tj
-34.7582 -1.3039 Td
(behavior)Tj
3.7133 0 Td
(in)Tj
1.0318 0 Td
(response)Tj
3.7361 0 Td
(to)Tj
1.0261 0 Td
(either)Tj
2.5285 0 Td
(changes)Tj
3.4015 0 Td
(in)Tj
1.0375 0 Td
(environmental)Tj
6.1115 0 Td
(conditions)Tj
4.4787 0 Td
(or)Tj
1.0941 0 Td
(internal)Tj
3.3619 0 Td
(physiological)Tj
-31.521 -1.2982 Td
(signals)Tj
2.9026 0 Td
(by)Tj
1.1792 0 Td
(modifying)Tj
4.3653 0 Td
(the)Tj
1.4684 0 Td
(duration)Tj
3.702 0 Td
(of)Tj
1.0148 0 Td
(component)Tj
4.8132 0 Td
(behavioral)Tj
4.3937 0 Td
(states.)Tj
-22.643 -1.2983 Td
(How)Tj
2.1826 0 Td
(the)Tj
1.4684 0 Td
(nervous)Tj
3.4129 0 Td
(system)Tj
2.9366 0 Td
(establishes)Tj
4.4334 0 Td
(the)Tj
1.4683 0 Td
(duration)Tj
3.702 0 Td
(of)Tj
1.0148 0 Td
(particular)Tj
4.1103 0 Td
(behavioral)Tj
4.3936 0 Td
(states)Tj
2.3925 0 Td
(and)Tj
1.7347 0 Td
(exe-)Tj
-34.4463 -1.3039 Td
(cutes)Tj
2.2506 0 Td
(transitions)Tj
4.4844 0 Td
(between)Tj
3.5263 0 Td
(them,)Tj
2.5172 0 Td
(however,)Tj
3.838 0 Td
(is)Tj
0.8448 0 Td
(largely)Tj
2.8629 0 Td
(unclear.)Tj
3.4186 0 Td
(Neuromodulators)Tj
7.387 0 Td
(such)Tj
2.058 0 Td
(as)Tj
1.0148 0 Td
(neuro-)Tj
-34.2026 -1.2983 Td
(peptides)Tj
3.5546 0 Td
(are)Tj
1.4513 0 Td
(attractive)Tj
3.9231 0 Td
(candidates)Tj
4.4617 0 Td
(for)Tj
1.389 0 Td
(orchestrating)Tj
5.5162 0 Td
(these)Tj
2.2507 0 Td
(processes)Tj
3.9685 0 Td
([)Tj
0.83 0.64 0.02 0 k
(6)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(].)Tj
3.0727 0 Td
(Pharmacological,)Tj
-29.5878 -1.2983 Td
(biochemical,)Tj
5.2894 0 Td
(and)Tj
1.7291 0 Td
(electrophysiological)Tj
8.141 0 Td
(studies)Tj
2.9821 0 Td
(have)Tj
2.0636 0 Td
(shed)Tj
2.0579 0 Td
(light)Tj
2.0239 0 Td
(on)Tj
1.2756 0 Td
(how)Tj
1.9389 0 Td
(neuropeptide)Tj
5.5729 0 Td
(signaling)Tj
-33.0744 -1.3039 Td
(can)Tj
1.627 0 Td
(modify)Tj
3.0841 0 Td
(the)Tj
1.4683 0 Td
(activity)Tj
3.1181 0 Td
(of)Tj
1.0148 0 Td
(neural)Tj
2.7723 0 Td
(circuits)Tj
3.1578 0 Td
([)Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(17)Tj
0 g
(].)Tj
3.5433 0 Td
(Neuropeptide)Tj
5.7599 0 Td
(signals)Tj
2.9027 0 Td
(typically)Tj
3.5659 0 Td
(activate)Tj
3.2542 0 Td
(or)Tj
-35.2684 -1.2982 Td
(refine)Tj
2.5398 0 Td
(patterns)Tj
3.4752 0 Td
(of)Tj
1.0148 0 Td
(neural)Tj
2.7723 0 Td
(activity)Tj
3.1124 0 Td
(that)Tj
1.7802 0 Td
(are)Tj
1.4513 0 Td
(generated)Tj
4.1329 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.474 0 Td
(actions)Tj
3.0558 0 Td
(of)Tj
1.0147 0 Td
(fast-acting)Tj
4.3881 0 Td
(transmitters)Tj
-32.4168 -1.2983 Td
(by)Tj
1.1792 0 Td
(altering)Tj
3.2768 0 Td
(cellular)Tj
3.1351 0 Td
(excitability)Tj
4.5751 0 Td
(or)Tj
1.0998 0 Td
(by)Tj
1.1792 0 Td
(modifying)Tj
4.3653 0 Td
(the)Tj
1.4684 0 Td
(efficiency)Tj
4.0308 0 Td
(of)Tj
1.0148 0 Td
(synaptic)Tj
3.5206 0 Td
(communication)Tj
-28.8451 -1.2983 Td
(between)Tj
3.5262 0 Td
(neurons.)Tj
3.7361 0 Td
(However,)Tj
4.0762 0 Td
(precisely)Tj
3.7077 0 Td
(linking)Tj
/F8 1 Tf
3.0614 0 Td
[()12()]TJ
1.0147 0 Td
[()-13()15()3()]TJ
/F7 1 Tf
1.8199 0 Td
(activation)Tj
4.1499 0 Td
(of)Tj
1.0148 0 Td
(specific)Tj
3.1918 0 Td
(neuromodulatory)Tj
-29.2987 -1.3039 Td
(systems)Tj
3.2995 0 Td
(with)Tj
1.9955 0 Td
(changes)Tj
3.4016 0 Td
(in)Tj
1.0375 0 Td
(circuit)Tj
2.8006 0 Td
(performance)Tj
5.3461 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(generation)Tj
4.5071 0 Td
(of)Tj
1.0148 0 Td
(alternate)Tj
3.702 0 Td
(behavioral)Tj
4.388 0 Td
(out-)Tj
-34.6901 -1.2982 Td
(comes)Tj
2.7552 0 Td
(remains)Tj
3.4412 0 Td
(a)Tj
0.6577 0 Td
(challenge.)Tj
4.1839 0 Td
(Uncovering)Tj
4.9663 0 Td
(basic)Tj
2.211 0 Td
(principles)Tj
4.1612 0 Td
(by)Tj
1.1849 0 Td
(which)Tj
2.6475 0 Td
(neuromodulators)Tj
7.2 0 Td
(influence)Tj
-33.4089 -1.2983 Td
(circuit)Tj
2.7949 0 Td
(activity)Tj
3.1181 0 Td
(and)Tj
1.7348 0 Td
(behavior)Tj
3.7077 0 Td
(in)Tj
1.0374 0 Td
(more)Tj
2.3414 0 Td
(simple)Tj
2.8574 0 Td
(systems)Tj
3.2995 0 Td
(provides)Tj
3.6453 0 Td
(an)Tj
1.2019 0 Td
(important)Tj
4.2859 0 Td
(framework)Tj
4.6148 0 Td
(for)Tj
-34.6391 -1.3039 Td
(tackling)Tj
3.3958 0 Td
(similar)Tj
2.9877 0 Td
(questions)Tj
4.0195 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(mammalian)Tj
5.0457 0 Td
(brain.)Tj
2.5738 0 Td
(In)Tj
1.1055 0 Td
(particular,)Tj
4.337 0 Td
(the)Tj
1.4683 0 Td
(neural)Tj
2.7723 0 Td
(circuit)Tj
2.8006 0 Td
(controlling)Tj
-33.012 -1.2983 Td
(egg-laying)Tj
4.3369 0 Td
(behavior)Tj
3.7134 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(nematode)Tj
/F8 1 Tf
4.2009 0 Td
[()-2( )6()2()6()11()8()6( )22(\r)4()12()14()7()-4(\n)]TJ
6.2022 0 Td
[()15()4()9()2( )16(\n)]TJ
/F7 1 Tf
2.999 0 Td
(provides)Tj
3.6454 0 Td
(a)Tj
0.6576 0 Td
(relatively)Tj
3.8381 0 Td
(simple)Tj
2.8573 0 Td
(and)Tj
-34.9566 -1.2983 Td
(experimentally)Tj
6.1568 0 Td
(tractable)Tj
3.6566 0 Td
(system)Tj
2.9367 0 Td
(to)Tj
1.0261 0 Td
(address)Tj
3.2258 0 Td
(these)Tj
2.2564 0 Td
(questions.)Tj
-18.0622 -1.3039 Td
(Under)Tj
2.8289 0 Td
(favorable)Tj
3.8891 0 Td
(conditions,)Tj
/F8 1 Tf
4.6998 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.0659 0 Td
[()15()4()9()2( )16(\n)]TJ
/F7 1 Tf
2.999 0 Td
(egg-laying)Tj
4.337 0 Td
(behavior)Tj
3.7133 0 Td
(cycles)Tj
2.5512 0 Td
(probabilistically)Tj
6.5763 0 Td
(between)Tj
-33.8567 -1.2982 Td
(two)Tj
1.7064 0 Td
(alternative)Tj
4.422 0 Td
(states:)Tj
2.6192 0 Td
(active)Tj
2.5228 0 Td
(phases)Tj
2.846 0 Td
(containing)Tj
4.5241 0 Td
(short)Tj
2.2847 0 Td
(bursts)Tj
2.6475 0 Td
(of)Tj
1.0148 0 Td
(egg-laying)Tj
4.3427 0 Td
(and)Tj
1.7291 0 Td
(prolonged)Tj
4.3313 0 Td
(peri-)Tj
-34.9906 -1.2983 Td
(ods)Tj
1.6157 0 Td
(of)Tj
1.0148 0 Td
(quiescence)Tj
4.5467 0 Td
(\(inactive)Tj
3.6794 0 Td
(phases\))Tj
3.1804 0 Td
([)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
(].)Tj
4.2463 0 Td
(This)Tj
1.9899 0 Td
(temporal)Tj
3.8381 0 Td
(organization)Tj
5.2781 0 Td
(of)Tj
1.0148 0 Td
(egg-laying)Tj
4.3426 0 Td
(allows)Tj
-34.7468 -1.3039 Td
(for)Tj
1.3832 0 Td
(spatial)Tj
2.778 0 Td
(dispersal)Tj
3.7304 0 Td
(of)Tj
1.0148 0 Td
(eggs,)Tj
2.1599 0 Td
(and)Tj
1.7292 0 Td
(may)Tj
1.9275 0 Td
(be)Tj
1.1509 0 Td
(beneficial)Tj
4.0478 0 Td
(for)Tj
1.389 0 Td
(survival)Tj
3.3335 0 Td
(of)Tj
1.0148 0 Td
(progeny)Tj
3.5093 0 Td
(by)Tj
1.1848 0 Td
(limiting)Tj
3.3959 0 Td
(local)Tj
-33.749 -1.2983 Td
(overcrowding)Tj
5.7713 0 Td
(and)Tj
1.7291 0 Td
(competition)Tj
5.0966 0 Td
(for)Tj
1.389 0 Td
(food.)Tj
2.2734 0 Td
(The)Tj
1.7801 0 Td
(core)Tj
1.9446 0 Td
(circuitry)Tj
3.6283 0 Td
(and)Tj
1.7291 0 Td
(signaling)Tj
3.8211 0 Td
(machinery)Tj
4.4901 0 Td
(control-)Tj
-33.6527 -1.2983 Td
(ling)Tj
1.7461 0 Td
(egg-laying)Tj
4.3426 0 Td
(is)Tj
0.8447 0 Td
(well)Tj
1.8199 0 Td
(described,)Tj
4.2803 0 Td
(but)Tj
1.5533 0 Td
(the)Tj
1.4684 0 Td
(mechanisms)Tj
5.2044 0 Td
(responsible)Tj
4.7508 0 Td
(for)Tj
1.389 0 Td
(this)Tj
1.6724 0 Td
(temporal)Tj
3.8381 0 Td
(organiza-)Tj
-32.91 -1.2982 Td
(tion)Tj
1.8425 0 Td
(have)Tj
2.0579 0 Td
(remained)Tj
4.0365 0 Td
(unclear.)Tj
3.4186 0 Td
(Egg-laying)Tj
4.4844 0 Td
(occurs)Tj
2.8346 0 Td
(when)Tj
2.4037 0 Td
(specialized)Tj
4.5241 0 Td
(sex-specific)Tj
4.7905 0 Td
(muscles)Tj
3.3846 0 Td
(contract,)Tj
-33.7774 -1.3039 Td
(opening)Tj
3.4979 0 Td
(the)Tj
1.4683 0 Td
(vulva)Tj
2.3358 0 Td
(and)Tj
1.7291 0 Td
(allowing)Tj
3.6226 0 Td
(for)Tj
1.3833 0 Td
(deposition)Tj
4.4447 0 Td
(of)Tj
1.0148 0 Td
(eggs.)Tj
2.16 0 Td
(Two)Tj
2.0239 0 Td
(serotonergic)Tj
5.1874 0 Td
(hermaphrodite-spe-)Tj
-28.8678 -1.2983 Td
(cific)Tj
1.8878 0 Td
(neurons)Tj
3.5093 0 Td
(\(HSN\))Tj
2.88 0 Td
(and)Tj
1.7291 0 Td
(two)Tj
1.7064 0 Td
(cholinergic)Tj
4.6942 0 Td
(ventral)Tj
2.9933 0 Td
(C)Tj
0.8844 0 Td
(class)Tj
2.0466 0 Td
(motor)Tj
2.7213 0 Td
(neurons)Tj
3.5093 0 Td
(\(VC4)Tj
2.3924 0 Td
(and)Tj
1.7291 0 Td
(VC5\))Tj
2.3981 0 Td
(make)Tj
-35.0813 -1.2983 Td
(excitatory)Tj
4.1612 0 Td
(synaptic)Tj
3.5263 0 Td
(contacts)Tj
3.5149 0 Td
(onto)Tj
2.0806 0 Td
(the)Tj
1.4684 0 Td
(Vm2)Tj
2.211 0 Td
(muscles)Tj
3.3788 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(].)Tj
2.0807 0 Td
(Egg-laying)Tj
4.4843 0 Td
(active)Tj
2.5229 0 Td
(states)Tj
2.3924 0 Td
(are)Tj
1.4513 0 Td
(associated)Tj
-33.2728 -1.3039 Td
(with)Tj
1.9955 0 Td
(serotonin)Tj
4.0535 0 Td
(release)Tj
2.9027 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.4684 0 Td
(HSNs)Tj
2.5625 0 Td
(that)Tj
1.7801 0 Td
(increases)Tj
3.8324 0 Td
(vulval)Tj
2.5796 0 Td
(muscle)Tj
3.0217 0 Td
(excitability)Tj
4.5751 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1A)Tj
0 g
(\))Tj
-32.7966 -1.2982 Td
([)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(].)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 133.6819 23.6976 re
W* n
q
133.6252 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 387.7228 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Neuromodu)Tj
5.1378 0 Td
(latory)Tj
2.6221 0 Td
(control)Tj
3.1606 0 Td
(of)Tj
1.0417 0 Td
(opposing)Tj
4.2024 0 Td
(behavio)Tj
3.43 0 Td
(ral)Tj
1.3039 0 Td
(states)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
95.5276 34.9228 m
251.8299 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(Genetics)Tj
4.1102 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4749 0 Td
(https://)Tj
2.9551 0 Td
(doi.org/10.13)Tj
5.7331 0 Td
(71/journal.p)Tj
5.1237 0 Td
(gen.100)Tj
3.5433 0 Td
(6697)Tj
0 g
3.1819 0 Td
(April)Tj
2.1756 0 Td
(6,)Tj
1.0418 0 Td
(2017)Tj
33.9451 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
31 0 obj
<>stream
endstream
endobj
32 0 obj
<>stream
endstream
endobj
33 0 obj
<>stream
endstream
endobj
34 0 obj
<>stream
endstream
endobj
35 0 obj
<>stream
endstream
endobj
36 0 obj
<>stream
endstream
endobj
37 0 obj
<>stream
endstream
endobj
38 0 obj
<>stream
endstream
endobj
39 0 obj
<>stream
endstream
endobj
40 0 obj
<>stream
endstream
endobj
41 0 obj
<>stream
endstream
endobj
42 0 obj
<>stream
endstream
endobj
43 0 obj
<>stream
endstream
endobj
44 0 obj
<>stream
BT
66.402 0 Td
(29)Tj
ET
Q
endstream
endobj
45 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 10 0 R/Annots 51 0 R/Contents 52 0 R/TrimBox[0 0 612 792]>>
endobj
50 0 obj
<>stream
JFIF C
$, !$4.763.22:ASF:=N>22HbINVX]^]8EfmeZlS[]Y C**Y;2;YYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYY
"
} !1AQa"q2#BR$3br
%&'()*456789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz
w !1AQaq"2B #3Rbr
$4%&'()*56789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz ? (
5hWѡv<ִ+&j