The human SWI/SNF complex associates with RUNX1 to control transcription of hematopoietic target genes
Authors
Bakshi, RachitHassan, Mohammad Q.
Pratap, Jitesh
Lian, Jane B.
Montecino, Martin A.
Van Wijnen, Andre J.
Stein, Janet L.
Imbalzano, Anthony N.
Stein, Gary S.
UMass Chan Affiliations
Department of Cell Biology and Cancer CenterDocument Type
Journal ArticlePublication Date
2010-05-28Keywords
Core Binding Factor Alpha 2 SubunitCore Binding Factor Alpha 1 Subunit
Hematopoietic Stem Cells
Transcription Factors
Cell Biology
Metadata
Show full item recordAbstract
The acute myeloid leukemia 1 (AML1, RUNX1) transcription factor is a key regulator of hematopoietic differentiation that forms multi-protein complexes with co-regulatory proteins. These complexes are assembled at target gene promoters in nuclear microenvironments to mediate phenotypic gene expression and chromatin related epigenetic modifications. Here, immunofluorescence microscopy and biochemical assays are used to show that RUNX1 associates with the human ATP-dependent SWI/SNF chromatin remodeling complex. The SWI/SNF subunits BRG1 and INI1 bind in vivo to RUNX1 target gene promoters (e.g., GMCSF, IL3, MCSF-R, MIP and p21). These interactions correlate with histone modifications characteristic of active chromatin, including acetylated H4 and dimethylated H3 Lysine 4. Down-regulation of RUNX1 by RNA interference diminishes the binding of BRG1 and INI1 at selected target genes. Taken together, our findings indicate that RUNX1 interacts with the human SWI/SNF complex to control hematopoietic-specific gene expression. J. Cell. Physiol. (c) 2010 Wiley-Liss, Inc.Source
J Cell Physiol. 2010 May 20. Link to article on publisher's siteDOI
10.1002/jcp.22240Permanent Link to this Item
http://hdl.handle.net/20.500.14038/26586PubMed ID
20506188Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/jcp.22240