Title

Ribonucleoprotein immunoprecipitation (RNP-IP): a direct in vivo analysis of microRNA-targets

UMMS Affiliation

Department of Cell Biology and Cancer Center

Date

6-22-2010

Document Type

Article

Subjects

Immunoprecipitation; Ribonucleoproteins; MicroRNAs

Abstract

We have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3'UTR derived primers. This flexible IP-based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time.

Rights and Permissions

Citation: J Cell Biochem. 2010 Jul 1;110(4):817-22. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

20564179

Journal Title

Journal of cellular biochemistry