Subnuclear targeting of the Runx3 tumor suppressor and its epigenetic association with mitotic chromosomes
Department of Cell Biology; Graduate School of Biomedical Sciences
Core Binding Factor Alpha 3 Subunit; Core Binding Factor alpha Subunits; Tumor Suppressor Proteins; Epigenesis, Genetic; Interphase; Mitosis; Nuclear Proteins
Runx proteins are tissue-specific transcriptional scaffolds that organize and assemble regulatory complexes at strategic sites of target gene promoters and at intranuclear foci to govern activation or repression. During interphase, fidelity of intranuclear targeting supports the biological activity of Runx1 and Runx2 proteins. Both factors regulate genes involved in cell cycle control and cell growth (e.g., rRNA genes), as well as lineage commitment. Here, we have examined the subcellular regulatory properties of the third Runx member, the tumor suppressor protein Runx3, during interphase and mitosis. Using in situ cellular and biochemical approaches we delineated a subnuclear targeting signal that directs Runx3 to discrete transcriptional foci that are nuclear matrix associated. Chromatin immunoprecipitation results show that Runx3 occupies rRNA promoters during interphase. We also find that Runx3 remains associated with chromosomes during mitosis and localizes with nucleolar organizing regions (NORs), reflecting an interaction with epigenetic potential. Taken together, our study establishes that common mechanisms control the subnuclear distribution and activities of Runx1, Runx2, and Runx3 proteins to support RNA polymerase I and II mediated gene expression during interphase and mitosis. J. Cell. Physiol. (c) 2008 Wiley-Liss, Inc.
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Citation: J Cell Physiol. 2008 Nov 12. Link to article on publisher's site
Journal of cellular physiology