Title
Tubulin requires tau for growth onto microtubule initiating sites
UMMS Affiliation
Department of Cell Biology
Date
11-1-1976
Document Type
Article
Subjects
Animals; Flagella; Glycoproteins; Kinetics; Microscopy, Electron; Microtubules; Molecular Weight; Proteins; Tubulin
Abstract
Tubulin purified by phosphocellulose chromatography and free of accessory proteins will not form microtubules in the absence or presence of microtubule initiating sites (flagellar microtubules). The capacity for growth onto pre-existing "seeds" can be restored by the addition of small quantities of partially purified tau protein. Larger quantities restore the capacity for spontaneous assembly. These results suggest that tubulin requires tau for both initiation and growth of microtubules and that tau is incorporated into the microtubule throughout its length.
Rights and Permissions
Citation: Proc Natl Acad Sci U S A. 1976 Nov;73(11):4070-4.
Related Resources
PubMed ID
1069293
Journal Title
Proceedings of the National Academy of Sciences of the United States of America