Title

Comparison of the microtubule proteins of neuroblastoma cells, brain, and Chlamydomonas flagella

UMMS Affiliation

Department of Cell Biology

Date

9-1-1971

Document Type

Article

Subjects

Algae, Green; Animals; Autoradiography; *Brain Chemistry; Carbon Isotopes; Chlamydomonas; Chromatography, DEAE-Cellulose; Densitometry; Electrophoresis, Disc; Flagella; Microscopy, Electron; Microtubules; Molecular Weight; Neoplasm Proteins; Nerve Tissue Proteins; Neuroblastoma; Proteins; Swine; Tritium

Abstract

Intact A microtubules isolated from outer doublet microtubules of Chlamydomonas flagella contain two separable proteins (tubulins) that differ in molecular weight and in amino-acid composition. The microtubule protein isolated from brain or neuroblastoma cells also has two electrophoretically distinct tubulins. Although the two tubulins of brain and neuroblastoma cells are electrophoretically similar to each other, only one of these tubulins migrates with the flagellar tubulins. This is the first evidence that (a) isolated, morphologically intact, single microtubules from flagella contain at least two different tubulins, and (b) at least one of these tubulins differs from tubulins that are isolated from other sources.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 1971 Sep;68(9):2273-7.

Related Resources

Link to Article in PubMed

Journal Title

Proceedings of the National Academy of Sciences of the United States of America

PubMed ID

5289385