The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex
Department of Cell Biology; Program in Molecular Medicine
Amino Acid Sequence; Animals; Base Sequence; Chlamydomonas reinhardtii; Cloning, Molecular; DNA, Complementary; DNA, Plant; Dynein ATPase; Flagella; Genes, Plant; Molecular Sequence Data; Mutagenesis, Insertional; Sequence Analysis, DNA
We have used an insertional mutagenesis/ gene tagging technique to generate new Chlamydomonas reinhardtii mutants that are defective in assembly of the uter ynein rm. Among 39 insertional oda mutants characterized, two are alleles of the previously uncloned ODA3 gene, one is an allele of the uncloned ODA10 gene, and one represents a novel ODA gene (termed ODA12). ODA3 is of particular interest because it is essential for assembly of both the outer dynein arm and the outer dynein arm docking complex (ODA-DC) onto flagellar doublet microtubules (Takada, S., and R. Kamiya. 1994. J. Cell Biol. 126:737- 745). Beginning with the inserted DNA as a tag, the ODA3 gene and a full-length cDNA were cloned. The cloned gene rescues the phenotype of oda3 mutants. The cDNA sequence predicts a novel 83. 4-kD protein with extensive coiled-coil domains. The ODA-DC contains three polypeptides; direct amino acid sequencing indicates that the largest of these polypeptides corresponds to ODA3. This protein is likely to have an important role in the precise positioning of the outer dynein arms on the flagellar axoneme.
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Citation: J Cell Biol. 1997 Jun 2;137(5):1069-80.
The Journal of cell biology
Koutoulis, Anthony; Pazour, Gregory J.; Wilkerson, Curtis G.; Inaba, Kazuo; Sheng, Hong; Takada, Saeko; and Witman, George B., "The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex" (1997). Cell and Developmental Biology Publications and Presentations. Paper 19.