UMMS Affiliation

Department of Cell and Developmental Biology



Document Type



Actins; Animals; Cell Adhesion; Cell Line, Tumor; Cell Membrane; Cell Nucleus; Cell Survival; Cytoskeleton; Elasticity; Fibroblasts; *Gene Expression Regulation; Green Fluorescent Proteins; *Homeostasis; Humans; Mice; Micromanipulation; Microscopy, Fluorescence; Microtubules; NIH 3T3 Cells; Nuclear Envelope; RNA, Small Interfering


How cells maintain nuclear shape and position against various intracellular and extracellular forces is not well understood, although defects in nuclear mechanical homeostasis are associated with a variety of human diseases. We estimated the force required to displace and deform the nucleus in adherent living cells with a technique to locally pull the nuclear surface. A minimum pulling force of a few nanonewtons--far greater than typical intracellular motor forces--was required to significantly displace and deform the nucleus. Upon force removal, the original shape and position were restored quickly within a few seconds. This stiff, elastic response required the presence of vimentin, lamin A/C, and SUN (Sad1p, UNC-84)-domain protein linkages, but not F-actin or microtubules. Although F-actin and microtubules are known to exert mechanical forces on the nuclear surface through molecular motor activity, we conclude that the intermediate filament networks maintain nuclear mechanical homeostasis against localized forces.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 2015 May 5;112(18):5720-5. doi: 10.1073/pnas.1502111112. Epub 2015 Apr 21. Link to article on publisher's site.


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Related Resources

Link to Article in PubMed


cytoskeleton, nuclear forces, nuclear mechanics, nuclear positioning, nuclear shape

Journal Title

Proceedings of the National Academy of Sciences of the United States of America

PubMed ID


Included in

Cell Biology Commons



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