Stable interaction between the products of the BRCA1 and BRCA2 tumor suppressor genes in mitotic and meiotic cells
Department of Cancer Biology
Antibodies, Monoclonal; BRCA1 Protein; BRCA2 Protein; Breast Neoplasms; Cell Line; Cell Nucleus; Chromosome Mapping; DNA Damage; DNA Repair; DNA Replication; DNA-Binding Proteins; Female; *Genes, BRCA1; *Genes, Tumor Suppressor; Humans; Meiosis; Mitosis; Neoplasm Proteins; Ovarian Neoplasms; Rad51 Recombinase; Transcription Factors; Transfection; Tumor Cells, Cultured; Zygote
BRCA1 and BRCA2 account for most cases of familial, early onset breast and/or ovarian cancer and encode products that each interact with hRAD51. Results presented here show that BRCA1 and BRCA2 coexist in a biochemical complex and colocalize in subnuclear foci in somatic cells and on the axial elements of developing synaptonemal complexes. Like BRCA1 and RAD51, BRCA2 relocates to PCNA+ replication sites following exposure of S phase cells to hydroxyurea or UV irradiation. Thus, BRCA1 and BRCA2 participate, together, in a pathway(s) associated with the activation of double-strand break repair and/or homologous recombination. Dysfunction of this pathway may be a general phenomenon in the majority of cases of hereditary breast and/or ovarian cancer.
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Citation: Mol Cell. 1998 Sep;2(3):317-28.