Carbohydrate-binding protein 35 is the major cell-surface laminin-binding protein in colon carcinoma
Department of Cancer Biology
Antigens, Differentiation; Carcinoma; Carrier Proteins; Chromatography, Affinity; Colonic Neoplasms; Flow Cytometry; Galectin 3; Hemagglutinins; Humans; Immunoblotting; Membrane Proteins; Neoplasm Proteins; Tumor Cells, Cultured
The interaction of tumor cells with laminin is thought to be critical in invasion and metastasis. We found that an endogenous lectin, carbohydrate-binding protein 35 (CBP-35), is the major laminin-binding protein on human colon carcinoma cells and that its surface expression suggests involvement in metastasis. We identified CBP-35 by laminin-affinity chromatography and immunoblotting. Surface expression of CBP-35 on eight human colon carcinoma cell lines was compared by flow cytometry. Poorly differentiated cell lines and DLD-2, a signet-ring carcinoma cell line, expressed more surface CBP-35 than well-differentiated cell lines. Poorly differentiated cell lines and DLD-2 are characterized as aggressive cell lines because they adhere to and invade through reconstituted basement membrane significantly better than well-differentiated cell lines. These data suggest that CBP-35 is involved in tumor cell-basement membrane interactions and that an increase in CBP-35 surface expression may facilitate metastatic potential of colon carcinoma cells.
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Citation: Arch Surg. 1991 Dec;126(12):1498-502.
Lee, Edward C.; Woo, Hee-Jong; Korzelius, Cynthia A.; Steele, Glenn D. Jr.; and Mercurio, Arthur M., "Carbohydrate-binding protein 35 is the major cell-surface laminin-binding protein in colon carcinoma" (1991). Cancer Biology Publications and Presentations. Paper 120.