Department of Cancer Biology
Animals; Basement Membrane; Cell Adhesion; Collagen; Fibronectins; Interferon-gamma; Laminin; Lipopolysaccharides; Macrophage Activation; Macrophages; Mice; Thioglycolates; Time Factors
The ability of thioglycollate (TG)-elicited peritoneal macrophages, a population of recently recruited monocytes, to adhere to the basement membrane glycoproteins laminin and type IV collagen is not a constitutive function of these cells. Adherence can be induced, however, by treatment with IFN-gamma and LPS. In general, IFN-gamma is more potent than LPS in promoting this adherence. Maximal adherence, however, is observed when IFN-gamma (greater than or equal to 5 U/ml) is used together with LPS (2.0 ng/ml). These requirements parallel the conditions needed to obtain tumoricidal activation of TG-elicited macrophages. Adherence to laminin, in the presence of these stimuli, is transient, being maximal at 8 h after their addition and diminishing with longer periods of incubation. In contrast, adherence to type IV collagen does not appear to be transient and IFN-gamma and LPS induce a more prolonged association of macrophages with this substratum.
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Citation: J Exp Med. 1989 Jan 1;169(1):303-8. Link to article on publisher's website
Shaw, Leslie M. and Mercurio, Arthur M., "Interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins" (1989). Cancer Biology Publications and Presentations. Paper 113.