UMMS Affiliation

Department of Cancer Biology

Date

11-1-1988

Document Type

Article

Subjects

Animals; Cell Adhesion; Cell Movement; Cytoskeleton; Electrophoresis, Polyacrylamide Gel; Extracellular Matrix; Female; Fibronectins; Laminin; Macrophages; Membrane Proteins; Mice; Mice, Inbred BALB C; Microscopy, Fluorescence; Precipitin Tests; Receptors, Immunologic; Receptors, Laminin; Tetradecanoylphorbol Acetate

Abstract

The ability of thioglycollate (TG)-elicited mouse peritoneal macrophages to adhere to a laminin substratum has been studied. These cells do not adhere to laminin-coated (20 micrograms/ml) surfaces, but the addition of phorbol myristate acetate (PMA; 50 ng/ml) results in their rapid adherence and spreading on this substratum. TG-elicited and PMA-activated macrophages, however, can bind soluble laminin. Macrophages adhere to fibronectin-coated surfaces and tissue culture plastic without PMA stimulation, and PMA does not increase the number of cells that adhere to these surfaces. The predominant surface proteins that bind specifically to laminin-Sepharose exhibit an Mr of 67 and 36 kD, but the expression of these proteins does not increase after PMA stimulation. Laminin receptor antibodies immunoprecipitate the 67-kD protein from radiolabled surface lysates and are capable of blocking macrophage adherence to a laminin substratum. Indirect immunofluorescence microscopy indicates that PMA stimulation does not increase receptor expression, but that it may induce the aggregation of the receptor on the cell surface. PMA stimulation also promotes macrophage spreading and induces a reorganization of the actin cytoskeleton. Taken together, these data indicate the mechanism by which PMA promotes macrophage adherence to laminin does not involve increased 67-kD receptor surface expression, but that it is related to the changes in cytoskeletal and receptor surface organization that occur in response to PMA stimulation.

Rights and Permissions

Citation: J Cell Biol. 1988 Nov;107(5):1873-80. Link to article on publisher's website

Related Resources

Link to Article in PubMed

PubMed ID

2972733

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.